Unmasking of deuterium kinetic isotope effects on the methane monooxygenase compound Q reaction by site-directed mutagenesis of component B

被引：64

作者：

Brazeau, BJ

Wallar, BJ

Lipscomb, JD ^{[1
]}

机构：

[1] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Ctr Met biocatalysis, Minneapolis, MN 55455 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2001年 / 123卷 / 42期

关键词：

D O I：

10.1021/ja016632i

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

引用

页码：10421 / 10422

页数：2

共 32 条

[1]

Bell RP., 1973, PROTON CHEM, V2nd

[2]

Brazeau B J, 2000, Subcell Biochem, V35, P233

[3] Kinetics and activation thermodynamics of methane monooxygenase compound Q formation and reaction with substrates [J].

Brazeau, BJ ;

Lipscomb, JD .

BIOCHEMISTRY, 2000, 39 (44) :13503-13515

[4] Solution structure of component B from methane monooxygenase derived through heteronuclear NMR and molecular modeling [J].

Chang, SL ;

Wallar, BJ ;

Lipscomb, JD ;

Mayo, KH .

BIOCHEMISTRY, 1999, 38 (18) :5799-5812

[5] Residues in Methylosinus trichosporium OB3b methane monooxygenase component B involved in molecular interactions with reduced- and oxidized-hydroxylase component:: A role for the N-terminus [J].

Chang, SL ;

Wallar, BJ ;

Lipscomb, JD ;

Mayo, KH .

BIOCHEMISTRY, 2001, 40 (32) :9539-9551

[6] RESOLUTION OF METHANE MONO-OXYGENASE OF METHYLOCOCCUS-CAPSULATUS-BATH INTO 3 COMPONENTS - PURIFICATION AND PROPERTIES OF COMPONENT-C, A-FLAVOPROTEIN [J].

COLBY, J ;

DALTON, H .

BIOCHEMICAL JOURNAL, 1978, 171 (02) :461-468

[7] Radiolytic reduction of methane monooxygenase dinuclear iron cluster at 77 K - EPR evidence for conformational change upon reduction or binding of component B to the diferric state [J].

Davydov, A ;

Davydov, R ;

Graslund, A ;

Lipscomb, JD ;

Andersson, KK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (11) :7022-7026

[8] An EPR study of the dinuclear iron site in the soluble methane monooxygenase from Methylococcus capsulatus (Bath) reduced by one electron at 77 K:: The effects of component interactions and the binding of small molecules to the diiron(III) center [J].

Davydov, R ;

Valentine, AM ;

Komar-Panicucci, S ;

Hoffman, BM ;

Lippard, SJ .

BIOCHEMISTRY, 1999, 38 (13) :4188-4197

[9]

Elango N, 1997, PROTEIN SCI, V6, P556

[10]

FOX BG, 1989, J BIOL CHEM, V264, P10023

← 1 2 3 4 →