Sequences within fibrinogen and intercellular adhesion molecule-1 (ICAM-1) modulate signals required for mitogenesis

The interaction of fibrinogen (Fg) with intercellular adhesion molecule-1 (ICAM) on B-lymphoid Raji cells results in mitogenesis (Gardiner, E. E., and D'Souza, S. E. (1997) J. Biol. Chem, 272, 15474-15480). Incubation of Raji with Fg resulted in the increased tyrosine phosphorylation of the receptor-associated tyrosine kinase, pp60(Src) and extracellular signal-regulated kinase-1 (ERK), The increase in ERK-1 phosphorylation was blocked by a peptide with sequence matching ICAM-1 (8-22) and corresponded to a decrease in ERK-1 enzymatic activity. 100 mu M amounts of Fg peptide gamma-(117-133) caused an increase in tyrosine phosphorylation of ERK-1, These results are consistent with our previous report wherein ICAM-1-(8-22) blocked Fg-induced mitogenesis and Fg-gamma-(117-133) induced proliferation in Raji, The specific inhibitor of MEK, PD98059 (25 mu M), abrogated the increased phosphorylation of ERK-1 and blocked Raji mitogenesis by >50%, Inhibitors of pp60(Src), geldanamycin (62 nm), and herbimycin A (2.5 mu M) blocked >50% of Raji proliferation. These results indicate that the proliferation induced by Fg interactions with ICAM-1 is mediated in part by receptor-associated tyrosine kinases and ERK-1, and that the recognition sequences within Fg and ICAM-1 participate in the signaling process.