Essential Role of DPPA3 for Chromatin Condensation in Mouse Oocytogenesis

被引：45

作者：

Liu, Yu-Jung ^{[1
]}

Nakamura, Toshinobu ^{[2
]}

Nakano, Toru ^{[1
,2
]}

机构：

[1] Osaka Univ, Grad Sch Frontier Biosci, Osaka, Japan

[2] Osaka Univ, Dept Pathol, Grad Sch Med, Osaka, Japan

来源：

BIOLOGY OF REPRODUCTION | 2012年 / 86卷 / 02期

关键词：

chromatin; female infertility; gamete biology; histone modifications; oocyte maturation; TRANSCRIPTIONAL ACTIVITY; DEVELOPMENTAL COMPETENCE; MEIOTIC COMPETENCE; OOCYTE GROWTH; SPECIFICATION; ORGANIZATION; KINETOCHORE; DYNAMICS; STELLA; GENOME;

D O I：

10.1095/biolreprod.111.095018

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dynamic alterations in chromatin configuration occur in mammalian oocytogenesis. Based on chromatin configuration patterns, fully grown oocytes are classified into two types. One is surrounded nucleolus (SN)-type and the other is nonsurrounded nucleolus (NSN)-type oocytes. Although chromatin condensation during the transition from NSN- to SN-type oocytes is a prerequisite for normal early embryonic development, the molecular mechanisms remain unclear. In this study, we analyzed the role of DPPA3 (also known as PGC7/Stella) in this transition using Dppa3-null oocytes. The NSN-to-SN transition was significantly impaired, and transcriptional repression was incomplete in the Dppa3-null oocytes. Additionally, we revealed that prior transcriptional repression was necessary for the NSN-to-SN transition. These findings demonstrate that DPPA3 is an essential factor for the production of functional oocytes through transcriptional repression and chromatin condensation.

引用

页数：8

共 23 条

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