Relationship between a stress membrane protein of Oenococcus oeni and glyceraldehyde-3-phosphate dehydrogenases

被引：8

作者：

Carreté, R

Reguant, C

Bordons, A

Constantí, M

机构：

[1] Univ Rovira & Virgili, Dept Bioquim & Biotecnol, Unitat Enol CeRTA, Tarragona 43007, Catalonia, Spain

[2] Univ Rovira & Virgili, Dept Engn Quim, ETSEQ, Tarragona 43006, Spain

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2005年 / 127卷 / 01期

关键词：

glyceraldehyde-3-phosphate dehydrogenase; malolactic fermentation; Oenococcus oeni; stress; SO2; wine;

D O I：

10.1385/ABAB:127:1:043

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The goal of this study was to analyze how the profiles of membrane proteins of Oenococcus oeni change under particular stress conditions of wine. Sodium dodecyl sulfate polyacrylamide gel electrophoresis protein profiles of membrane fraction showed that a 40-kDa protein was overexpressed in the presence of SO2. The sequence of its N-terminal fragment showed a significant identity with glyceraldehyde-3-phosphate dehydrogenases (GAPDHs), but the protein showed no GAPDH activity. This sequence was compared with those of other GAPDHs with ClustalW alignment, and it was found to be somewhat similar to that of the cell-wall and membrane proteins of other lactic acid bacteria.

引用

页码：43 / 51

页数：9

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