Induction of in vitro angiogenesis in the endothelial-derived cell line, EA hy926, by ethanol is mediated through PKC and MAPK

被引:52
作者
Jones, MK [1 ]
Sarfeh, IJ
Tarnawski, AS
机构
[1] Dept Vet Affairs Med Ctr, Dept Med, Long Beach, CA 90822 USA
[2] Dept Vet Affairs Med Ctr, Dept Surg, Long Beach, CA 90822 USA
[3] Univ Calif Irvine, Irvine, CA 92717 USA
关键词
protein kinase C; mitogen activated protein; kinase; ERK; MEK; signaling; kinase inhibitors; kinase activation;
D O I
10.1006/bbrc.1998.9095
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously shown that ethanol-induced injury to the gastric mucosa triggers increased expression of the angiogenic factors, basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) and angiogenesis. To further investigate ethanol-induced angiogenesis, we used an in vitro angiogenesis model which employs the ability of an endothelial-derived cell line (EA hy926) to form tubelike structures resembling capillaries when plated on the matrix material, Matrigel. We report that serum-starved EA hy926 cells, incubated for as little as 5 minutes with ethanol concentrations of 1.0-2.5%, formed tubelike structures reflecting in vitro angiogenesis. Control cells, not incubated with ethanol, did not form tubelike structures. incubation for 5 minutes with 2.5% ethanol resulted in increased activities of PKC and MAP kinase (ERK2) by 1.6-fold (p < 0.05) and 2.3-fold (P < 0.001), respectively. Futhermore, inhibitors of the MAPK kinase, MEK (PD98059) and PRC (GF 109203X) prevented the induction of in vitro angiogenesis by ethanol, (C) 1998 Academic Press.
引用
收藏
页码:118 / 123
页数:6
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