The cotton rat: An underutilized animal model for human infectious diseases can now be exploited using specific reagents to cytokines, chemokines, and interferons

被引:38
作者
Blanco, JCG
Pletneva, L
Boukhvalova, M
Richardson, JY
Harris, KA
Prince, GA
机构
[1] Virion Syst Inc, Rockville, MD 20850 USA
[2] Uniformed Serv Univ Hlth Sci, Dept Pediat, Bethesda, MD 20814 USA
关键词
D O I
10.1089/107999004772719873
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cotton rat represents the best or only animal model for a large number of human infectious diseases, and it may be unique among small laboratory animals in its susceptibility to several potential agents of bioterrorism. Although the cotton rat is a reliable model to define pathologic changes produced during infection with human pathogens, the lack of specific reagents has precluded a more extensive analysis of the molecular basis of pathogenesis. Here, we report the cloning of 24 cotton rat genes encoding various cytokines, chemokines, and interferons (IFNs). Analysis of the expression of most of these genes was performed by RT-PCR in cotton rat macrophages during treatment with lipopolysaccharide (LPS) and in cotton rat lungs after infection with influenza virus. The availability of these reagents will provide the tools for molecular analysis of pathogenesis and immune responses to a wide variety of pathogens and set the basis for the development of new prophylactic and therapeutic strategies against human infectious diseases.
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页码:21 / 28
页数:8
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