Induction of heme oxygenase 1 in liver of spontaneously diabetic rats

被引：27

作者：

Cosso, L

Maineri, EP

Traverso, N

Rosatto, N

Pronzato, MA

Cottalasso, D

Marinari, UM

Odetti, P

机构：

[1] Univ Genoa, Sect Gen Pathol, Dept Expt Med, DIMES, I-16132 Genoa, Italy

[2] Univ Genoa, Dept Internal Med, I-16132 Genoa, Italy

来源：

FREE RADICAL RESEARCH | 2001年 / 34卷 / 02期

关键词：

oxidative stress; heme oxygenase; RT/PCR; stress protein;

D O I：

10.1080/10715760100300171

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been suggested that diabetes induces an increase in oxidative stress; the increased expression of heme-oxygenase 1 (HO-1) in liver is believed to be a sensitive marker of the stress response. The aim of this study was to examine whether diabetes is able to induce HO-1 expression in liver. The specific mRNA was amplified by RT/PCR and calibrated with amplified beta -actin mRNA. The mRNA HO-1 let els in the liver of spontaneously diabetic rats were increased by 1.8 fold compared with non diabetics; this supports the hypothesis of weak but significant oxidative damage due to chronic hyperglycaemia. This work represents the first in vivo study exploring the semi-quantitative expression of HO-1 in the liver of spontaneously diabetic rats.

引用

页码：189 / 191

页数：3

共 12 条

[1] ROLE OF OXIDATIVE STRESS IN DEVELOPMENT OF COMPLICATIONS IN DIABETES [J].