Reduction of cerebral infarction in rats by biliverdin associated with amelioration of oxidative stress

被引：62

作者：

Deguchi, Kentaro ^{[1
,2
]}

Hayashi, Takeshi ^{[1
]}

Nagotani, Shoko ^{[1
]}

Sehara, Yoshihide ^{[1
]}

Zhany, HanZhe ^{[1
]}

Tsuhciya, Atsushi ^{[1
]}

Ohta, Yasuyuki ^{[1
]}

Tomiyama, Koji ^{[2
]}

Morimoto, Nobutoshi ^{[1
]}

Miyazaki, Masahiro ^{[2
]}

Huh, Nam-Ho ^{[2
]}

Nakao, Atsunori ^{[3
]}

Kamiya, Tatsushi ^{[1
]}

Abe, Koji ^{[1
]}

机构：

[1] Okayama Univ, Dept Neurol, Grad Sch Med, Okayama 7008558, Japan

[2] Okayama Univ, Dept Cell Biol, Grad Sch Med Dent & Pharm, Okayama, Japan

[3] Univ Pittsburgh, Dept Surg, Tomas E Starzl Transplant Inst, Pittsburgh, PA USA

来源：

BRAIN RESEARCH | 2008年 / 1188卷

关键词：

Biliverdin; brain ischemia; free radical scavenger; oxidative stress;

D O I：

10.1016/j.brainres.2007.07.104

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Biliverdin (BV), one of the byproducts of heme catalysis through heme oxygenase (HO) system, is a scavenger of reactive oxygen species (ROS). We hypothesized that BV treatment could protect rat brain cells from oxidative injuries via its anti-oxidant efficacies. Cerebral infarction was induced by transient middle cerebral artery occlusion (tMCAO) for 90 min, followed by reperfusion. BV or vehicle was administered intraperitoneally immediately after reperfusion. The size of the cerebral infarction 2 days after tMCAO was evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) stain. Superoxide generation 4 h after tMCAO was determined by detection of oxidized hydroethidine. In addition, the oxidative impairment of neurons were immunohistochemically assessed by stain for lipid peroxidation with 4-hydroxy-2-nonenal (4-HNE) and damaged DNA with 8-hydroxy-2'-deoxyguanosine (8-OHdG). BV treatment significantly reduced infarct volume of the cerebral cortices associated with less superoxide production and decreased oxidative injuries of brain cells. The present study demonstrated that treatment with BV ameliorated the oxidative injuries on neurons and decreased brain infarct size in rat tMCAO model. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1 / 8

页数：8

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