Polyelectrolyte Multilayer Nanofilms Used as Thin Materials for Cell Mechano-Sensitivity Studies

被引：41

作者：

Boudou, Thomas ^{[1
,2
]}

Crouzier, Thomas ^{[1
,2
]}

Nicolas, Claire ^{[1
,2
]}

Ren, Kefeng ^{[1
,2
]}

Picart, Catherine ^{[1
,2
]}

机构：

[1] Minatec, Grenoble Inst Technol, F-38016 Grenoble, France

[2] Minatec, LMGP, F-38016 Grenoble, France

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 01期

关键词：

cell adhesion and spreading; cross-linking; fibers; layer-by-layer assembly; mechanical properties; mechano-sensitivity; ATOMIC-FORCE MICROSCOPY; MECHANICAL-PROPERTIES; IN-VITRO; BIOMEDICAL APPLICATIONS; SUBSTRATE MECHANICS; ENDOTHELIAL-CELLS; FOCAL ADHESIONS; YOUNGS MODULUS; CROSS-LINKING; GEL STIFFNESS;

D O I：

10.1002/mabi.201000301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Three types of multilayer films made from poly(L-lysine)/hyaluronan, chitosan/hyaluronan, and poly(allylamine hydrochloride)/poly(L-glutamic acid), were used to investigate the interplay between film mechano-chemical properties and cell adhesion. We showed that C2C12 myoblast adhesion and proliferation depended on the extent of film cross-linking for all films whatever their internal chemistry. Cell spreading areas were found to correlate with the film's stiffness and to be distributed over a unique curve. Immuno-staining of the cytoskeletal components revealed the formation of F-actin stress fibers and vinculin plaques only on stiff films. Finally, we compared our results with previous studies performed on polyacrylamide and PDMS gels, two recognized materials for mechano-sensitivity studies. We found that the effect of substrate stiffness on cell spreading is material-dependent.

引用

页码：77 / 89

页数：13

共 69 条

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Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures [J].