Assessing Gut Microbial Diversity from Feces and Rectal Mucosa

被引:146
作者
Durban, Ana [1 ,2 ,3 ]
Abellan, Juan J. [1 ,2 ,3 ]
Jimenez-Hernandez, Nuria [1 ,2 ,3 ]
Ponce, Marta [4 ,5 ]
Ponce, Julio [4 ,5 ]
Sala, Teresa [4 ,5 ]
D'Auria, Giuseppe [1 ,2 ,3 ]
Latorre, Amparo [1 ,2 ,3 ]
Moya, Andres [1 ,2 ,3 ]
机构
[1] Univ Valencia, Inst Cavanilles Biodiversidad & Biol Evolut, Valencia 46071, Spain
[2] CSISP, Valencia 46020, Spain
[3] CIBEResp, Barcelona, Spain
[4] Hosp Univ La Fe, Serv Med Digest, Valencia 46009, Spain
[5] Ctr Invest Biomed Enfermedades Hepat & Digest CIB, Barcelona, Spain
关键词
GASTROINTESTINAL-TRACT; DATABASE;
D O I
10.1007/s00248-010-9738-y
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071301 [植物生态学];
摘要
Gut microbiota is the most complex bacterial community in the human body and its study may give important clues to the etiology of different intestinal diseases. Most studies carried out so far have used fecal samples, assuming that these samples have a similar distribution to the communities present throughout the colon. The present study was designed to test this assumption by comparing samples from the rectal mucosa and feces of nine healthy volunteers by sequencing libraries of 16S rRNA genes. At the family taxonomic level, where rarefaction curves indicate that the observed number of taxa is close to the expected one, we observe under different statistical analyses that fecal and mucosal samples cluster separately. The same is found at the level of species considering phylogenetic information. Consequently, it cannot be stated that both samples from a given individual are of similar composition. We believe that the evidence in support of this statement is strong and that it would not change by increasing the number of individuals and/or performing massive sequencing. We do not expect clinicians to stop using feces for research, but we think it is important to caution them on their potential lack of representativeness with respect to the bacterial biofilm on the rectal mucosa.
引用
收藏
页码:123 / 133
页数:11
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