Protective effect of decorin on acute ischaemia-reperfusion injury in the rat kidney

被引:33
作者
Alan, Cabir [1 ]
Kocoglu, Hasan [2 ]
Altintas, Ramazan [3 ]
Alici, Bulent [3 ]
Ersay, Ahmet Resit [1 ]
机构
[1] Canakkale Onsekiz Mart Univ, Dept Urol, Fac Med, Canakkale, Turkey
[2] Canakkale Mil Hosp, Dept Urol, Canakkale, Turkey
[3] Istanbul Univ, Dept Urol, Cerrahpasa Fac Med, Istanbul, Turkey
关键词
transforming growth factor-beta 1; decorin; glomerulosclerosis; GROWTH-FACTOR-BETA; ACUTE-RENAL-FAILURE; EXTRACELLULAR-MATRIX; TRANSFORMING GROWTH-FACTOR-BETA-1; PROTEOGLYCAN DECORIN; TGF-BETA; EXPRESSION; PROLIFERATION; DIFFERENTIATION; SUPPRESSION;
D O I
10.5114/aoms.2011.22069
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Introduction: Transforming growth factor-beta 1 (TGF-beta 1) has a crucial role in collagen synthesis and fibrosis. TGF-beta 1 can be antagonized and/or reduced by the action of certain agents. We propose to identify the role of decorin in treatment of tubular and interstitial fibrosis and in the inhibition of TGF-beta 1 in an acute ischaemic kidney. Material and methods: We grouped 34 female Sprague Dawley type rats into 3 groups as 9 sham, 9 ischaemia-reperfusion (I/R) and 16 I/R + decorin respectively. The rats in the I/R + decorin group had decorin administered intraperitoneally at the dose of 0.1 mg/kg for 9 days after reperfusion. After 9 days, all the rats in the 3 groups were unilaterally nephrectomized. The TGF-beta 1 level was measured immunohistochemically in the nephrectomized material. Results: The TGF-beta 1 level was lower in the I/R + decorin group. Evaluation of apoptotic activity level by caspase staining showed a statistically significant difference between the 3 groups. The number of caspase stained cells was lower in the I/R + decorin group. The amount of collagen in interstitial tissue was higher in the I/R group than in the I/R + decorin group, but this difference was not statistically significant. Conclusions: We found that the TOE-Ill level the so-called initiator of fibrotic activity and apoptotic activity were low in the I/R + decorin group. Additional studies must be performed to understand the role of decorin in inhibition of TGF-beta 1 and to assess decorin's routine use in acute renal ischaemia.
引用
收藏
页码:211 / 216
页数:6
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