Proteomic identification of glyceraldehyde 3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis

被引:174
作者
Hancock, JT [1 ]
Henson, D [1 ]
Nyirenda, M [1 ]
Desikan, R [1 ]
Harrison, J [1 ]
Lewis, M [1 ]
Hughes, J [1 ]
Neill, SJ [1 ]
机构
[1] Univ W England, Fac Sci Appl, Genom Res Inst, Ctr Res Plant Sci, Bristol BS16 1QY, Avon, England
基金
英国生物技术与生命科学研究理事会;
关键词
Arabidopsis thaliana; glyceraldehyde 3-phosphate dehydrogenase; hydrogen peroxide; proteomics; reactive oxygen species signalling;
D O I
10.1016/j.plaphy.2005.07.012
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Hydrogen peroxide (H2O2) is now recognised as a key signalling molecule in eukaryotes. In plants, H2O2 is involved in regulating stomatal closure, gravitropic responses, gene expression and programmed cell death. Although several kinases, such as oxidative signal-inducible 1 (OXI1) kinase and mitogen-activated protein kinases are known to be activated by exogenous H2O2, little is known about the proteins that directly react with H2O2. Here, we utilised a proteomic approach, using iodoacetamide-based fluorescence tagging of proteins in conjunction with mass spectrometric analysis, to identify several proteins that might be potential targets of H2O2 in the cytosolic fraction of Arabidopsis thaliana, the most prominent of which was cytosolic glyceraldehyde 3-phosphate dehydrogenase (cGAPDH; EC 1.2.1.12). cGAPDH from Arabidopsis is inactivated by H2O2 in vitro, and this inhibition is reversible by the subsequent addition of reductants such as reduced glutathione (GSH). It has been suggested recently that Arabidopsis GAPDH has roles outside of its catalysis as part of glycolysis, while in other systems this includes that of mediating reactive oxygen species (ROS) signalling. Here, we suggest that cGAPDH in Arabidopsis might also have such a role in mediating ROS signalling in plants. (c) 2005 Elsevier SAS. All rights reserved.
引用
收藏
页码:828 / 835
页数:8
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