PPARγ activators down-regulate the expression of PPARγ in 3T3-L1 adipocytes

被引：54

作者：

Camp, HS

Whitton, AL

Tafuri, SR

机构：

[1] Warner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Dept Biol Mol, Ann Arbor, MI 48105 USA

[2] Warner Lambert Parke Davis, Parke Davis Pharmaceut Res Div, Dept Cell Biol, Ann Arbor, MI 48105 USA

来源：

FEBS LETTERS | 1999年 / 447卷 / 2-3期

关键词：

PPAR; desensitization; troglitazone; anti-diabetic;

D O I：

10.1016/S0014-5793(99)00268-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcriptional activation of PPAR gamma by the anti-diabetic compound troglitazone enhances the rate of 3T3-L1 adipocyte differentiation. In this study, we examined the effects of troglitazone, a specific PPAR gamma ligand, on the expression of PPAR gamma during and after 3T3-L1 adipocyte differentiation. Troglitazone treatment caused a significant decrease in PPAR gamma proteins and DNA binding activity. This reduction was associated with a similar decrease in transcription of PPAR gamma mRNA, These data suggest that in 3T3-L1 cells, the expression of PPAR gamma is auto-regulated. (C) 1999 Federation of European Biochemical Societies.

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页码：186 / 190

页数：5

相关论文

共 27 条

[1] CLONING OF A PROTEIN THAT MEDIATES TRANSCRIPTIONAL EFFECTS OF FATTY-ACIDS IN PREADIPOCYTES - HOMOLOGY TO PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS [J].

AMRI, EZ ;

BONINO, F ;

AILHAUD, G ;

ABUMRAD, NA ;

GRIMALDI, PA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (05) :2367-2371

[2]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[3] LIGAND-DEPENDENT DOWN-REGULATION OF STABLY TRANSFECTED HUMAN GLUCOCORTICOID RECEPTORS IS ASSOCIATED WITH THE LOSS OF FUNCTIONAL GLUCOCORTICOID RESPONSIVENESS [J].

BELLINGHAM, DL ;

SAR, M ;

CIDLOWSKI, JA .

MOLECULAR ENDOCRINOLOGY, 1992, 6 (12) :2090-2102

[4]

Camp HS, 1997, J BIOL CHEM, V272, P10811

[5] PEROXISOME PROLIFERATOR AND RETINOID SIGNALING PATHWAYS COREGULATE PREADIPOCYTE PHENOTYPE AND SURVIVAL [J].

CHAWLA, A ;

LAZAR, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (05) :1786-1790

[6] A SINGLE POLYPEPTIDE POSSESSES THE BINDING AND TRANSCRIPTION ACTIVITIES OF THE ADENOVIRUS MAJOR LATE TRANSCRIPTION FACTOR [J].

CHODOSH, LA ;

CARTHEW, RW ;

SHARP, PA .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (12) :4723-4733

[7] DIFFERENTIATION-INDUCED GENE-EXPRESSION IN 3T3-L1 PREADIPOCYTES - CCAAT ENHANCER BINDING-PROTEIN INTERACTS WITH AND ACTIVATES THE PROMOTERS OF 2 ADIPOCYTE-SPECIFIC GENES [J].

CHRISTY, RJ ;

YANG, VW ;

NTAMBI, JM ;

GEIMAN, DE ;

LANDSCHULZ, WH ;

FRIEDMAN, AD ;

NAKABEPPU, Y ;

KELLY, TJ ;

LANE, MD .

GENES & DEVELOPMENT, 1989, 3 (09) :1323-1335

[8] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[9]

FANG C, 1993, BIOCHEM BIOPH RES CO, V196, P671

[10] A DIRECT ROLE FOR C/EBP AND THE AP-I-BINDING SITE IN GENE-EXPRESSION LINKED TO ADIPOCYTE DIFFERENTIATION [J].

HERRERA, R ;

RO, HS ;

ROBINSON, GS ;

XANTHOPOULOS, KG ;

SPIEGELMAN, BM .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (12) :5331-5339