Influence of postprandial triglyceride-rich lipoproteins on lipid-mediated gene expression in smooth muscle cells of the human coronary artery

被引：64

作者：

Bermudez, Beatriz ^{[1
]}

Lopez, Sergio ^{[1
]}

Pacheco, Yolanda M. ^{[1
]}

Villar, Jose ^{[2
]}

Muriana, Francisco J. G. ^{[1
]}

Hoheisel, Joeerg D. ^{[3
]}

Bauer, Andrea ^{[3
]}

Abia, Rocio ^{[1
]}

机构：

[1] Inst Grasa CSIC, Seville 41012, Spain

[2] Hosp Univ Virgen Rocio, Serv Internal Med, Seville, Spain

[3] German Canc Res Ctr, Dept Funct Genome Anal, D-6900 Heidelberg, Germany

来源：

CARDIOVASCULAR RESEARCH | 2008年 / 79卷 / 02期

关键词：

postprandial lipoproteins; gene array analysis; coronary disease; fatty acids; smooth muscle cells;

D O I：

10.1093/cvr/cvn082

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Aims Postprandial triglyceride-rich lipoproteins (TRL) have a direct effect on vascular smooth muscle cells (SMC) and they increase the risk of atherogenesis. Here, we have tested the hypothesis that the different fatty acid composition of TRL is capable of differentially modifying gene expression in human coronary artery SMC (CASMC). In addition, the effect of TRL on cell proliferation and transcription factor activation was also evaluated. Methods and results TRL were prepared from plasma of healthy volunteers after the ingestion of meals enriched in refined olive oil (ROO), butter or a mixture of vegetable and fish oils (VEFO). We use cDNA microarrays to determine the genes differentially expressed in TRL-treated CASMC. Correspondence cluster analysis demonstrated that TRL-butter, -ROO and -VEFO provoked different transcriptional pro. files in CASMC. Sixty-six genes were regulated by TRL-butter, 55 by -ROO, and 47 by -VEFO. The data revealed that TRL-butter predominantly activated genes involved in the regulation of cell proliferation and inflammation. Likewise, TRL-VEFO induced the expression of genes implicated in inflammation, while TRL-ROO promoted a less atherogenic gene profile. Conclusion The pathophysiological contribution of TRL to the development of atherosclerosis and the stability of atherosclerotic plaques may depend on the fatty acid composition of TRL. Our findings suggest a role for macrophage-inhibiting cytokine-1 (MIC-1) in coronary artery cardiovascular events.

引用

页码：294 / 303

页数：10

共 33 条

[1] Postprandial triacylglycerols from dietary virgin olive oil are selectively cleared in humans [J].