Structural basis for DNA bridging by barrier-to-autointegration factor

被引：87

作者：

Bradley, CM ^{[1
]}

Ronning, DR ^{[1
]}

Ghirlando, R ^{[1
]}

Craigie, R ^{[1
]}

Dyda, F ^{[1
]}

机构：

[1] NIDDKD, Mol Biol Lab, US NIH, Bethesda, MD 20892 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2005年 / 12卷 / 10期

关键词：

D O I：

10.1038/nsmb989

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of barrier-to-autointegration factor (BAF) to bind and bridge DNA in a sequence-independent manner is crucial for its role in retroviral integration and a variety of cellular processes. To better understand this behavior, we solved the crystal structure of BAF bound to DNA. The structure reveals that BAF bridges DNA using two pairs of helix-hairpin-helix motifs located on opposite surfaces of the BAF dimer without changing its conformation.

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页码：935 / 936

页数：2

相关论文

共 16 条

[1]

Brown P. O., 1997, P161

[2] Solution structure of the cellular factor BAF responsible for protecting retroviral DNA from autointegration [J].

Cai, M ;

Huang, Y ;

Zheng, R ;

Wei, SQ ;

Ghirlando, R ;

Lee, MS ;

Craigie, R ;

Gronenborn, AM ;

Clore, GM .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (10) :903-909

[3] The barrier-to-autointegration protein is a host factor for HIV type 1 integration [J].

Chen, HM ;

Engelman, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15270-15274

[4] The helix-hairpin-helix DNA-binding motif: A structural basis for non-sequence-specific recognition of DNA [J].

Doherty, AJ ;

Serpell, LC ;

Ponting, CP .

NUCLEIC ACIDS RESEARCH, 1996, 24 (13) :2488-2497

[5] Structural basis for removal of adenine mispaired with 8-oxoguanine by MutY adenine DNA glycosylase [J].

Fromme, JC ;

Banerjee, A ;

Huang, SJ ;

Verdine, GL .

NATURE, 2004, 427 (6975) :652-656

[6] Structure of a trapped endonuclease III-DNA covalent intermediate [J].

Fromme, JC ;

Verdine, GL .

EMBO JOURNAL, 2003, 22 (13) :3461-3471

[7] Barrier-to-autointegration factor plays crucial roles in cell cycle progression and nuclear organization in Drosophila [J].

Furukawa, K ;

Sugiyama, S ;

Osouda, S ;

Goto, H ;

Inagaki, M ;

Horigome, T ;

Omata, S ;

McConnell, M ;

Fisher, PA ;

Nishida, Y .

JOURNAL OF CELL SCIENCE, 2003, 116 (18) :3811-3823

[8] DNA bending and a flip-out mechanism for base excision by the helix-hairpin-helix DNA glycosylase, Escherichia coli AlkA [J].

Hollis, T ;

Ichikawa, Y ;

Ellenberger, T .

EMBO JOURNAL, 2000, 19 (04) :758-766

[9] A previously unidentified host protein protects retroviral DNA from autointegration [J].

Lee, MS ;

Craigie, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (04) :1528-1533

[10] The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes [J].

Lin, CW ;

Engelman, A .

JOURNAL OF VIROLOGY, 2003, 77 (08) :5030-5036