Fluorescent europium-modified polymer nanoparticles for rapid and sensitive anthrax sensors

被引：90

作者：

Oh, Wan-Kyu ^{[1
]}

Jeong, Yoon Seon ^{[1
]}

Song, Jooyoung ^{[1
]}

Jang, Jyongsik ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Biol Engn, World Class Univ WCU Program Chem Convergence Ene, Sch Chem & Biol Engn, Seoul 151742, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 29卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Bacillus anthracis; Europium; Polyacrylonitrile; Nanoparticles; Fluorescence; SURFACE MODIFICATION; LUMINESCENCE; BIOMARKER; NANOMATERIALS; FABRICATION; NANOFIBERS;

D O I：

10.1016/j.bios.2011.08.013

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Novel fluorescent polyacrylonitrile nanoparticles were synthesized by microemulsion polymerization and Schiff base modification. By further modification with europium, the polyacrylonitrile nanoparticles could be used as a highly sensitive and rapid sensor for Bacillus anthracis spore detection in aqueous solution. The europium-modified polyacrylonitrile nanoparticles were readily combined with dipicolinic acid as a unique biomarker of B. anthracis, leading to high fluorescence emission. These nanoparticles enabled ratiometric detection without instrument-specific calibration due to the internal fluorescence reference. Additionally, the europium-modified polyacrylonitrile nanoparticle sensors exhibited a remarkable limit of detection (10 pM) for dipicolinic acid and outstanding selectivity (160x) over aromatic ligands in aqueous solution. The ultrafine nanoparticle sensor showed a high capability for detecting anthrax due to the increased surface area-to-volume ratio and enhanced dispersibility. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：172 / 177

页数：6

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