Multiple stress signal integration in the regulation of the complex σS-dependent csiD-ygaF-gabDTP operon in Escherichia coli

被引:51
作者
Metzner, M [1 ]
Germer, J [1 ]
Hengge, R [1 ]
机构
[1] Free Univ Berlin, Inst Biol, D-14195 Berlin, Germany
关键词
D O I
10.1046/j.1365-2958.2003.03867.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The csiD-ygaF-gabDTP region in the Escherichia coli genome represents a cluster of sigma(S)-controlled genes. Here, we investigated promoter structures, sigma factor dependencies, potential co-regulation and environmental regulatory patterns for all of these genes. We find that this region constitutes a complex operon with expression being controlled by three differentially regulated promoters: (i) csiD(p), which affects the expression of all five genes, is cAMP-CRP/sigma(S)-dependent and activated exclusively upon carbon starvation and stationary phase; (ii) gabD(p1), which is sigma(S)-dependent and exhibits multiple stress induction like sigma(S) itself; and (iii) gabD(p2)[previously suggested by Schneider, B.L., Ruback, S., Kiupakis, A.K., Kasbarian, H., Pybus, C., and Reitzer, L. (2002) J. Bacteriol. 184: 6976-6986], which appears to be Nac/sigma(70)-controlled and to respond to poor nitrogen sources. In addition, we identify a novel repressor, CsiR, which modulates csiD(p) activity in a temporal manner during early stationary phase. Finally, we propose a physiological role for sigma(S)-controlled GabT/D-mediated gamma-aminobutyrate (GABA) catabolism and glutamate accumulation in general stress adaptation. This physiological role is reflected by the activation of the operon-internal gabD(p1) promoter under the different conditions that also induce sigma(S), which include shifts to acidic pH or high osmolarity as well as starvation or stationary phase.
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页码:799 / 811
页数:13
相关论文
共 52 条
[1]   Escherichia coli genes regulated by cell-to-cell signaling [J].
Baca-DeLancey, RR ;
South, MMT ;
Ding, XD ;
Rather, PN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (08) :4610-4614
[2]   MOLECULAR ANALYSIS OF 2 GENES OF THE ESCHERICHIA-COLI GAB CLUSTER - NUCLEOTIDE-SEQUENCE OF THE GLUTAMATE SUCCINIC SEMIALDEHYDE TRANSAMINASE GENE (GABT) AND CHARACTERIZATION OF THE SUCCINIC SEMIALDEHYDE DEHYDROGENASE GENE (GABD) [J].
BARTSCH, K ;
VONJOHNNMARTEVILLE, A ;
SCHULZ, A .
JOURNAL OF BACTERIOLOGY, 1990, 172 (12) :7035-7042
[3]  
Becker G, 2001, MOL MICROBIOL, V39, P1153, DOI 10.1046/j.1365-2958.2001.02313.x
[4]   Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase [J].
Capitani, G ;
De Biase, D ;
Aurizi, C ;
Gut, H ;
Bossa, F ;
Grütter, MG .
EMBO JOURNAL, 2003, 22 (16) :4027-4037
[5]   TRANSPOSITION AND FUSION OF LAC GENES TO SELECTED PROMOTERS IN ESCHERICHIA-COLI USING BACTERIOPHAGE-LAMBDA AND BACTERIOPHAGE-MU [J].
CASADABAN, MJ .
JOURNAL OF MOLECULAR BIOLOGY, 1976, 104 (03) :541-555
[6]   Control of acid resistance in Escherichia coli [J].
Castanie-Cornet, MP ;
Penfound, TA ;
Smith, D ;
Elliott, JF ;
Foster, JW .
JOURNAL OF BACTERIOLOGY, 1999, 181 (11) :3525-3535
[7]   Escherichia coli acid resistance:: cAMP receptor protein and a 20 bp cis-acting sequence control pH and stationary phase expression of the gadA and gadBC glutamate decarboxylase genes [J].
Castanie-Cornet, MP ;
Foster, JW .
MICROBIOLOGY-UK, 2001, 147 :709-715
[8]   THE ACCUMULATION OF GLUTAMATE IS NECESSARY FOR OPTIMAL-GROWTH OF SALMONELLA-TYPHIMURIUM IN MEDIA OF HIGH-OSMOLALITY BUT NOT INDUCTION OF THE PROU OPERON [J].
CSONKA, LN ;
IKEDA, TP ;
FLETCHER, SA ;
KUSTU, S .
JOURNAL OF BACTERIOLOGY, 1994, 176 (20) :6324-6333
[9]   One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].
Datsenko, KA ;
Wanner, BL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645
[10]   The response to stationary-phase stress conditions in Escherichia coli:: role and regulation of the glutamic acid decarboxylase system [J].
De Biase, D ;
Tramonti, A ;
Bossa, F ;
Visca, P .
MOLECULAR MICROBIOLOGY, 1999, 32 (06) :1198-1211