Clinical study of cultured epithelial autografts in liquid suspension in severe burn patients

被引:41
作者
Yim, Haejun
Yang, Hyeong Tae
Cho, Yong Suk
Seo, Cheong Hoon [2 ]
Lee, Boung Chul [3 ]
Ko, Jang Hyu [4 ]
Kwak, In Suk [5 ]
Kim, Dohern
Hur, Jun
Kim, Jong Hyun
Chun, Wook [1 ]
机构
[1] Hallym Univ, Hangang Sacred Heart Hosp, Med Ctr, Dept Burn Surg,Burn Ctr, Seoul 150719, South Korea
[2] Hallym Univ, Hangang Sacred Heart Hosp, Med Ctr, Dept Rehabil Med, Seoul 150719, South Korea
[3] Hallym Univ, Hangang Sacred Heart Hosp, Med Ctr, Dept Psychiat, Seoul 150719, South Korea
[4] Hallym Univ, Hangang Sacred Heart Hosp, Med Ctr, Dept Plast Surg, Seoul 150719, South Korea
[5] Hallym Univ, Hangang Sacred Heart Hosp, Med Ctr, Dept Anesthesiol, Seoul 150719, South Korea
关键词
CEA (cultured epithelial autograft); Extensive burn; Acellular dermal matrix; SKIN SUBSTITUTES; RESUSCITATION; KERATINOCYTES;
D O I
10.1016/j.burns.2011.03.018
中图分类号
R4 [临床医学];
学科分类号
100218 [急诊医学];
摘要
We address the clinical application of the suspension type cultured epithelial autografts (CEAs), Keraheal (TM) (MCTT, Seoul, Korea), along with the effects, application method, merits and demerits thereof. From February 2007 to June 2010, 29 burn patients with extensive burns, participated in the suspension type of CEA clinical test. A widely meshed autograft (1:4-6 ratio) was applied to the wound bed and the suspension type CEA was sprayed with a Tissomat cell sprayer, followed by a Tissucol spray, a fibrin sealant. The patients' (men/women = 26/3) median (interquartile ranges) age was 42 (30-49) years old, the burned TBSA was 55 (44 60) %, and the full thickness burn area was 40 (30-46.5) %. The area of Keraheal (TM) applied was 800(400-1200) cm(2). The take rate was 96(90.5-99) % and 100(98.5-100) % at 2 and 4 weeks after treatment with Keraheal (TM), respectively. The Vancouver burn scar scale was 5 (4-6.5), 4 (3-6), and 3 (2-4) at 8, 12 and 24 weeks after the Keraheal (TM) application. Widely meshed autograft must be applied in massive burns but it's take rate is greatly reduced. The CEAs enhance the take rate of a wide meshed autograft in massive burns and allow for grafting wide meshed autograft together with acellular dermal matrix in some cases. (C) 2011 Elsevier Ltd and ISBI. All rights reserved.
引用
收藏
页码:1067 / 1071
页数:5
相关论文
共 10 条
[1]
BAXTER CR, 1978, SURG CLIN N AM, V58, P1313
[2]
Boyce ST, 2006, J TRAUMA, V60, P821, DOI 10.1097/01.ta.0000196802.91829.cc
[3]
Widely meshed autograft associated with cultured autologous epithelium for the treatment of major burns in children: Report of 12 cases [J].
Braye, F ;
Oddou, L ;
Bertin-Maghit, M ;
Belgacem, S ;
Damour, O ;
Spitalier, P ;
Guillot, M ;
Bouchard, C ;
Gueugniaud, PY ;
Goudeau, M ;
Petit, P ;
Tissot, E .
EUROPEAN JOURNAL OF PEDIATRIC SURGERY, 2000, 10 (01) :35-40
[4]
INFLUENCES OF DIFFERENT RESUSCITATION REGIMENS ON ACUTE EARLY WEIGHT-GAIN IN EXTENSIVELY BURNED PATIENTS [J].
DU, GB ;
SLATER, H ;
GOLDFARB, IW .
BURNS, 1991, 17 (02) :147-150
[5]
Optimized delivery of skin keratinocytes by aerosolization and suspension in fibrin tissue adhesive [J].
Harkin, DG ;
Dawson, RA ;
Upton, Z .
WOUND REPAIR AND REGENERATION, 2006, 14 (03) :354-363
[6]
Tissue engineering of cultured skin substitutes [J].
Horch, RE ;
Kopp, J ;
Kneser, U ;
Beier, J ;
Bach, AD .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2005, 9 (03) :592-608
[7]
Assessment of adhesion assays for use with keratinocytes [J].
May, AL ;
Wood, FM ;
Stoner, ML .
EXPERIMENTAL DERMATOLOGY, 2001, 10 (01) :62-69
[8]
The use of cultured epithelial autograft in the treatment of major burn injuries: A critical review of the literature [J].
Wood, F. M. ;
Kolybaba, M. L. ;
Allen, P. .
BURNS, 2006, 32 (04) :395-401
[9]
The use of cultured epithelial autograft in the treatment of major burn wounds: Eleven years of clinical experience [J].
Wood, F. M. ;
Kolybaba, M. L. ;
Allen, P. .
BURNS, 2006, 32 (05) :538-544
[10]
Treatment of burns and chronic wounds using a new cell transfer dressing for delivery of autologous keratinocytes [J].
Zhu N. ;
Warner R.M. ;
Simpson C. ;
Glover M. ;
Hernon C.A. ;
Kelly J. ;
Fraser S. ;
Brotherston T.M. ;
Ralston D.R. ;
MacNeil S. .
European Journal of Plastic Surgery, 2005, 28 (5) :319-330