Properties of TAXI-type endoxylanase inhibitors

被引：101

作者：

Gebruers, K

Brijs, K

Courtin, CM

Fierens, K

Goesaert, H

Rabijns, A

Raedschelders, G

Robben, J

Sansen, S

Sorensen, JF

Van Campenhout, S

Delcour, JA

机构：

[1] Katholieke Univ Leuven, Food Chem Lab, B-3001 Louvain, Belgium

[2] Katholieke Univ Leuven, Lab Analyt Chem & Med Physicochem, B-3000 Louvain, Belgium

[3] Katholieke Univ Leuven, Lab Gene Technol, B-3001 Louvain, Belgium

[4] Danisco, Enzyme Dev, DK-8220 Brabrand, Denmark

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2004年 / 1696卷 / 02期

关键词：

endoxylanase; endoxylanase inhibitor; TAXI; cereal; protein; property;

D O I：

10.1016/j.bbapap.2003.08.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two types of proteinaceous endoxylanase inhibitors occur in different cereals, i.e. the TAXI [Triticum aestivum endoxylanase inhibitor]type and XIP [endoxylanase inhibiting protein]-type inhibitors. The present paper focuses on the TAXI-type proteins and deals with their structural characteristics and the identification, characterisation and heterologous expression of a TAXI gene from wheat. In addition, to shed light on the mechanism by which TAXI-type endoxylanase inhibitors work, the enzyme specificity, the optimal conditions for maximal inhibition activity, the molar complexation ratio and the inhibition kinetics of the inhibitors are explained and the effect of mutations of an endoxylanase on the inhibition by TAXIs is discussed. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：213 / 221

页数：9

共 76 条

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