The Kluyveromyces lactis γ-toxin targets tRNA anticodons

被引：174

作者：

Lu, J ^{[1
]}

Huang, B ^{[1
]}

Esberg, A ^{[1
]}

Johansson, MJO ^{[1
]}

Byström, AS ^{[1
]}

机构：

[1] Umea Univ, Dept Mol Biol, S-90187 Umea, Sweden

来源：

RNA | 2005年 / 11卷 / 11期

关键词：

K. lactis gamma-toxin; tRNA endonuclease; ELP3; TRM9; 5-methoxycarbonylmethyl-2-thiouridine;

D O I：

10.1261/rna.2172105

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(mcm)(s)(UUC)(5)(2)(Glu), tRNA(mcm5s2UUU)(Lys), and tRNA(mcm)(s)(UUG)(5)(2)(Gln) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (MCMIS2 U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and kti11-kti13 mutants. The K. lactis gamma-toxin is the first eukaryotic toxin shown to target tRNA.

引用

页码：1648 / 1654

页数：7

共 38 条

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