Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus

被引：10

作者：

Verhaest, M

Van den Ende, W

Yoshida, M

Le Roy, K

Peeraer, Y

Sansen, S

De Ranter, CJ

Van Laere, A

Rabijns, A

机构：

[1] Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Analyt Chem & Med Fysicochem, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven, Dept Biol, Fac Wetenschappen, Lab Mol Plantenfysiol, B-3001 Heverlee, Belgium

[3] Natl Agr Res Ctr Hokkaido Reg, Sapporo, Hokkaido 0628555, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2004年 / 60卷

关键词：

D O I：

10.1107/S0907444903029317

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fructan 1-exohydrolase IIa (1-FEH IIa), a plant enzyme involved in fructan breakdown, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals are tetragonal, belonging to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a=139.83, b=139.83, c=181.94 Angstrom. Calculation of the Matthews coefficient indicates there to be two or three molecules in the asymmetric unit. Synchrotron radiation was used to collect a complete native data set to a resolution of 2.35 Angstrom.

引用

页码：553 / 554

页数：2

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