The ATM-related cofactor Tra1 is a component of the purified SAGA complex

被引：162

作者：

Grant, PA ^{[1
]}

Schieltz, D

Pray-Grant, MG

Yates, JR

Workman, JL

机构：

[1] Penn State Univ, Howard Hughes Med Inst, University Pk, PA 16802 USA

[2] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA

[3] Univ Washington, Ctr Comprehens Biol, Seattle, WA 98195 USA

来源：

MOLECULAR CELL | 1998年 / 2卷 / 06期

关键词：

D O I：

10.1016/S1097-2765(00)80300-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The SAGA histone acetyltransferase/transcriptional adaptor complex is composed of multiple transcriptional regulators including Ada, Spt, and TAF(II) proteins. Here we identify an additional navel subunit of the complex, Tra1, an ATM/PI-3-kinase-related homolog of the human TRRAP cofactor, which is essential for c-Myc and E2F-mediated oncogenic transformation. Mass spectrometry, immunoblotting, and immunoprecipitation experiments confirm the stable association of this protein within SAGA, in addition, the Tra1 protein is a component of at least two other histone acetyltransferase protein complexes. These results indicate a role for Tra1 In the regulation of transcriptional activation through the recruitment of HAT activity to an activator-bound promoter.

引用

页码：863 / 867

页数：5

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