Microelectronic DNA assay for the detection of BRCA1 gene mutations

被引：12

作者：

Chen, H

Han, J

Li, J

Meyyappan, M

机构：

[1] NASA, Ames Res Ctr, Moffett Field, CA 94035 USA

[2] ELORET, Moffett Field, CA 94035 USA

来源：

BIOMEDICAL MICRODEVICES | 2004年 / 6卷 / 01期

基金：

美国国家航空航天局;

关键词：

microelectronic array; mutations; nanoelectronic array; cancer detection; carbon nanotubes; molecular diagnosis;

D O I：

10.1023/B:BMMD.0000013366.85609.dd

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Mutations in BRCA1 are characterized by predisposition to breast cancer, ovarian cancer and prostate cancer as well as colon cancer. Prognosis for this cancer survival depends upon the stage at which cancer is diagnosed. Reliable and rapid mutation detection is crucial for the early diagnosis and treatment. We developed an electronic assay for the detection of a representative single nucleotide polymorphism (SNP), deletion and insertion in BRCA1 gene by the microelectronics microarray instrumentation. The assay is rapid, and it takes 30 minutes for the immobilization of target DNA samples, hybridization, washing and readout. The assay is multiplexing since it is carried out at the same temperature and buffer conditions for each step. The assay is also highly specific, as the signal-to-noise ratio is much larger than recommended value (72.86 to 321.05 vs. 5) for homozygotes genotyping, and signal ratio close to the perfect value 1 for heterozygotes genotyping (1.04).

引用

页码：55 / 60

页数：6

共 21 条

[1] Automated detection of prevalent mutations in BRCA1 and BRCA2 genes, using a fluorogenic PCR allelic discrimination assay [J].

Abbaszadegan, MR ;

Struewing, JP ;

Brown, KM ;

Snider, JV ;

Goodsaid, F ;

Gore-Langton, R ;

Hughes, MR .

GENETIC TESTING, 1997, 1 (03) :171-180

[2] MUTATIONS IN THE BRCA1 GENE IN FAMILIES WITH EARLY-ONSET BREAST AND OVARIAN-CANCER [J].

CASTILLA, LH ;

COUCH, FJ ;

ERDOS, MR ;

HOSKINS, KF ;

CALZONE, K ;

GARBER, JE ;

BOYD, J ;

LUBIN, MB ;

DESHANO, ML ;

BRODY, LC ;

COLLINS, FS ;

WEBER, BL .

NATURE GENETICS, 1994, 8 (04) :387-391

[3]

Coleman MP, 2000, LANCET, V356, P590, DOI 10.1016/S0140-6736(00)02593-9

[4]

COTRAN RS, 1999, NEOPLASIA PATHOLOGIC, P292

[5]

Den Dunnen JT, 1999, HUM MUTAT, V14, P95, DOI 10.1002/(SICI)1098-1004(1999)14:2<95::AID-HUMU1>3.3.CO

[6]

2-7

[7]

EASTON DF, 1995, AM J HUM GENET, V56, P265

[8]

FRIEDMAN LS, 1995, AM J HUM GENET, V57, P1284

[9]

HIGGINSON J, 1992, CAMBRIDGE MONOGRAPHS, P377

[10] RAPID DETECTION OF BRCA1 MUTATIONS BY THE PROTEIN TRUNCATION TEST [J].

HOGERVORST, FBL ;

CORNELIS, RS ;

BOUT, M ;

VANVLIET, M ;

OOSTERWIJK, JC ;

OLMER, R ;

BAKKER, B ;

KLIJN, JGM ;

VASEN, HFA ;

MEIJERSHEIJBOER, H ;

MENKO, FH ;

CORNELISSE, CJ ;

DENDUNNEN, JT ;

DEVILEE, P ;

VANOMMEN, GJB .

NATURE GENETICS, 1995, 10 (02) :208-212

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