A member of the short-chain dehydrogenase/reductase (SDR) superfamily is a target of the ecdysone response in honey bee (Apis mellifera) caste development

被引：20

作者：

Guidugli, KR

Hepperle, C

Hartfelder, K ^{[1
]}

机构：

[1] Univ Sao Paulo, FFCLRP, Dept Biol, BR-14040901 Ribeirao Preto, Brazil

[2] Univ Sao Paulo, FMRP, Dept Genet, BR-14040900 Ribeirao Preto, Brazil

[3] Univ Tubingen, Inst Zool, D-72076 Tubingen, Germany

来源：

APIDOLOGIE | 2004年 / 35卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

Apis mellifera; differential display PCR; ecdysteroid; alcohol dehydrogenase; caste polyphenism;

D O I：

10.1051/apido:2003068

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Many aspects in caste polyphenism result from hormonally controlled differential gene expression. A DDRT-PCR screen for ecdysteroid-regulated genes in ovaries revealed a set of ESTs coding for metabolic enzymes. For a cDNA encoding a short-chain dehydrogenase/reductase (SDR) we obtained the complete coding sequence (246 amino acids), revealing the protein motifs typical of insect SDRs. Its initially high expression in early fifth-instar larvae vanished in prepupae. Expression levels in worker larvae were higher than in queen larvae, suggesting negative regulation by the caste-specific ecdysteroid titer. This finding was confirmed by in vitro exposure of competent worker ovaries to makisterone A. In contrast to whole body RNA extracts, two SDR transcripts were detected in the ovaries. Both had their expression downregulated by makisterone A. Hormonal regulation and tissue-specific expression pattern makes this SDR an interesting enzyme for comparative molecular studies on social insect caste polyphenisms.

引用

页码：37 / 47

页数：11

共 40 条

[1] The genome sequence of Drosophila melanogaster [J].

Adams, MD ;

Celniker, SE ;

Holt, RA ;

Evans, CA ;

Gocayne, JD ;

Amanatides, PG ;

Scherer, SE ;

Li, PW ;

Hoskins, RA ;

Galle, RF ;

George, RA ;

Lewis, SE ;

Richards, S ;

Ashburner, M ;

Henderson, SN ;

Sutton, GG ;

Wortman, JR ;

Yandell, MD ;

Zhang, Q ;

Chen, LX ;

Brandon, RC ;

Rogers, YHC ;

Blazej, RG ;

Champe, M ;

Pfeiffer, BD ;

Wan, KH ;

Doyle, C ;

Baxter, EG ;

Helt, G ;

Nelson, CR ;

Miklos, GLG ;

Abril, JF ;

Agbayani, A ;

An, HJ ;

Andrews-Pfannkoch, C ;

Baldwin, D ;

Ballew, RM ;

Basu, A ;

Baxendale, J ;

Bayraktaroglu, L ;

Beasley, EM ;

Beeson, KY ;

Benos, PV ;

Berman, BP ;

Bhandari, D ;

Bolshakov, S ;

Borkova, D ;

Botchan, MR ;

Bouck, J ;

Brokstein, P .

SCIENCE, 2000, 287 (5461) :2185-2195

[2]

ANDRES AJ, 1992, DEVELOPMENT, V116, P865

[3] THE DROSOPHILA E93 GENE FROM THE 93F EARLY PUFF DISPLAYS STAGE-SPECIFIC AND TISSUE-SPECIFIC REGULATION BY 20-HYDROXYECDYSONE [J].

BAEHRECKE, EH ;

THUMMEL, CS .

DEVELOPMENTAL BIOLOGY, 1995, 171 (01) :85-97

[4] Genesis of Drosophila ADH:: the shaping of the enzymatic activity from a SDR ancestor [J].

Benach, J ;

Atrian, S ;

Ladenstein, R ;

Gonzàlez-Duarte, R .

CHEMICO-BIOLOGICAL INTERACTIONS, 2001, 130 (1-3) :405-415

[5] THE MESSENGER-RNA FOR ALCOHOL-DEHYDROGENASE IN DROSOPHILA-MELANOGASTER DIFFERS IN ITS 5' END IN DIFFERENT DEVELOPMENTAL STAGES [J].

BENYAJATI, C ;

SPOEREL, N ;

HAYMERLE, H ;

ASHBURNER, M .

CELL, 1983, 33 (01) :125-133

[6]

Butler SJ, 1997, DEVELOPMENT, V124, P781

[7] Juvenile hormone effect on DNA synthesis and apoptosis in caste-specific differentiation of the larval honey bee (Apis mellifera L.) ovary [J].

Capella, ICS ;

Hartfelder, K .

JOURNAL OF INSECT PHYSIOLOGY, 1998, 44 (5-6) :385-391

[8]

Corona M, 1999, J EXP BIOL, V202, P929

[9] Mitochondrial genes as sites of primary action of steroid hormones [J].

Demonacos, CV ;

Karayanni, N ;

Hatzoglou, E ;

Tsiriyiotis, C ;

Spandidos, DA ;

Sekeris, CE .

STEROIDS, 1996, 61 (04) :226-232

[10] Peroxisome proliferator-activated receptors: Nuclear control of metabolism [J].

Desvergne, B ;

Wahli, W .

ENDOCRINE REVIEWS, 1999, 20 (05) :649-688

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