Candida albicans Csy1p is a nutrient sensor important for activation of amino acid uptake and hyphal morphogenesis

被引:58
作者
Brega, E
Zufferey, R
Ben Mamoun, C
机构
[1] Univ Connecticut, Ctr Hlth, Ctr Microbial Pathogenesis, Farmington, CT 06030 USA
[2] Univ Connecticut, Ctr Hlth, Dept Genet & Dev Biol, Farmington, CT 06030 USA
[3] Univ Connecticut, Ctr Hlth, Dept Pathol, Farmington, CT 06030 USA
关键词
D O I
10.1128/EC.3.1.135-143.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Candida albicans is an important human pathogen that displays a remarkable ability to detect changes in its environment and to respond appropriately by changing its cell morphology and physiology. Serum- and amino acid-based media are known to induce filamentous growth in this organism. However, the mechanism by which amino acids induce filamentation is not yet known. Here, we describe the identification and characterization of the primary amino acid sensor of C albicans, Csy1. We show that Csy1p plays an important role in amino acid sensing and filamentation. Loss of Csy1p results in a lack of amino acid-mediated activation of amino acid transport and a lack of induction of transcription of specific amino acid permease genes. Furthermore, a csy1Delta/csy1Delta strain, lacking Csy1p, is defective in filamentation and displays altered colony morphology in serum- and amino acid-based media. These data provide the first evidence that C. albicans utilizes the amino acid sensor Csy1p to probe its environment, coordinate its nutritional requirements, and determine its morphological state.
引用
收藏
页码:135 / 143
页数:9
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