Molecular genetic analysis of isolated single cells and other minute DNA samples is limited because there is insufficient DNA to perform more than one independent PCR amplification. One solution to this problem is to first amplify the entire genome, thus providing enough DNA for numerous subsequent PCRs. In this study we have investigated four different methods of whole genome amplification performed on single cells, and have identified a protocol that generates sufficient quantities of DNA for comparative genomic hybridisation (CGH) as well as more than 90 independent amplification reactions. Thus, numerous specific loci and the copy number of every chromosome can be assessed in a single cell. We report here the first reliable application of CGH to single cells from human preimplantation embryos (blastomeres) and to single fibroblasts, buccal cells and amniocytes.
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Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
Ao, A
;
Wells, D
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Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
Wells, D
;
Handyside, AH
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Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
Handyside, AH
;
Winston, RML
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Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
Winston, RML
;
Delhanty, JDA
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Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
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WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
KALLIONIEMI, A
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KALLIONIEMI, OP
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WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
KALLIONIEMI, OP
;
SUDAR, D
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WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
SUDAR, D
;
RUTOVITZ, D
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WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
RUTOVITZ, D
;
GRAY, JW
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WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
GRAY, JW
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WALDMAN, F
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WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
WALDMAN, F
;
PINKEL, D
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WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
机构:
Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
Ao, A
;
Wells, D
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
Wells, D
;
Handyside, AH
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
Handyside, AH
;
Winston, RML
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
Winston, RML
;
Delhanty, JDA
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, EnglandUniv London Univ Coll, Galton Lab, Human Genet Grp, London NW1 2HE, England
机构:
WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
KALLIONIEMI, A
;
KALLIONIEMI, OP
论文数: 0引用数: 0
h-index: 0
机构:
WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
KALLIONIEMI, OP
;
SUDAR, D
论文数: 0引用数: 0
h-index: 0
机构:
WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
SUDAR, D
;
RUTOVITZ, D
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h-index: 0
机构:
WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
RUTOVITZ, D
;
GRAY, JW
论文数: 0引用数: 0
h-index: 0
机构:
WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
GRAY, JW
;
WALDMAN, F
论文数: 0引用数: 0
h-index: 0
机构:
WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND
WALDMAN, F
;
PINKEL, D
论文数: 0引用数: 0
h-index: 0
机构:
WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLANDWESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND