The ELF4-ELF3-LUX complex links the circadian clock to diurnal control of hypocotyl growth

被引:696
作者
Nusinow, Dmitri A. [1 ,2 ]
Helfer, Anne [1 ,2 ]
Hamilton, Elizabeth E. [1 ,2 ]
King, Jasmine J. [1 ,2 ]
Imaizumi, Takato [1 ]
Schultz, Thomas F. [1 ]
Farre, Eva M. [1 ]
Kay, Steve A. [1 ,2 ]
机构
[1] Univ Calif San Diego, Div Biol Sci, Sect Cell & Dev Biol, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Ctr Chronobiol, La Jolla, CA 92093 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
QUANTITATIVE-ANALYSIS; ARTIFICIAL MICRORNAS; FUNCTIONAL GENOMICS; PROTEIN; ELF3; TRANSCRIPTION; COMPONENT; ENCODES; TIME; COINCIDENCE;
D O I
10.1038/nature10182
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The circadian clock is required for adaptive responses to daily and seasonal changes in environmental conditions(1-3). Light and the circadian clock interact to consolidate the phase of hypocotyl cell elongation to peak at dawn under diurnal cycles in Arabidopsis thaliana(4-7). Here we identify a protein complex (called the evening complex)-composed of the proteins encoded by EARLY FLOWERING 3 (ELF3), ELF4 and the transcription-factor-encoding gene LUX ARRHYTHMO (LUX; also known as PHYTOCLOCK 1)-that directly regulates plant growth(8-12). ELF3 is both necessary and sufficient to form a complex between ELF4 and LUX, and the complex is diurnally regulated, peaking at dusk. ELF3, ELF4 and LUX are required for the proper expression of the growth-promoting transcription factors encoded by PHYTOCHROME INTERACTING FACTOR 4 (PIF4) and PIF5 (also known as PHYTOCHROME INTERACTING FACTOR 3-LIKE 6) under diurnal conditions(4,6,13). LUX targets the complex to the promoters of PIF4 and PIF5 in vivo. Mutations in PIF4 and/or PIF5 are epistatic to the loss of the ELF4-ELF3-LUX complex, suggesting that regulation of PIF4 and PIF5 is a crucial function of the complex. Therefore, the evening complex underlies the molecular basis for circadian gating of hypocotyl growth in the early evening.
引用
收藏
页码:398 / U161
页数:7
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