A novel polyherbal microbicide with inhibitory effect on bacterial, fungal and viral genital pathogens

被引：49

作者：

Talwar, G. P. ^{[1
]}

Dar, Sajad A. ^{[1
]}

Rai, Mahendra K. ^{[1
,2
]}

Reddy, K. V. R. ^{[3
]}

Mitra, Debashis ^{[4
]}

Kulkarni, Sujata V. ^{[4
]}

Doncel, Gustavo F. ^{[5
]}

Buck, Christopher B. ^{[6
]}

Schiller, John T. ^{[6
]}

Muralidhar, Sumathi ^{[7
]}

Bala, Manju ^{[7
]}

Agrawal, S. S. ^{[2
]}

Bansal, Kavita ^{[1
]}

Verma, Jitendra K. ^{[1
]}

机构：

[1] Talwar Res Fdn, New Delhi 110068, India

[2] Univ Delhi, Delhi Inst Pharmaceut Sci & Res, New Delhi, India

[3] Natl Inst Res Reprod Hlth ICMR, Bombay, Maharashtra, India

[4] Natl Ctr Cell Sci, Pune, Maharashtra, India

[5] Eastern Virginia Med Sch, CONRAD, Arlington, VA USA

[6] NCI, NIH, Bethesda, MD 20892 USA

[7] Safdarjang Hosp, Reg STD Teaching Training & Res Ctr, New Delhi, India

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2008年 / 32卷 / 02期

关键词：

Basant; Neisseria gonorrhoeae; Candida; HIV; HPV;

D O I：

10.1016/j.ijantimicag.2008.03.004

中图分类号：

R51 [传染病];

学科分类号：

100401 [流行病与卫生统计学];

摘要：

A polyherbal cream (Basant) has been formulated using diferuloylmethane (curcumin), purified extracts of Emblica officinalis (Amla), purified saponins from Sapindus mukorossi, Aloe vera and rose water along with pharmacopoeially approved excipients and preservatives. Basant inhibits the growth of WHO strains and clinical isolates of Neisseria gonorrhoeae, including those resistant to penicillin, tetracycline, nalidixic acid and ciprofloxacin. It has pronounced inhibitory action against Candida glabrata, Candida albicans and Candida tropicalis isolated from women with vulvovaginal candidiasis, including three isolates resistant to azole drugs and amphotericin B. Basant displayed a high virucidal action against human immunodeficiency virus HIV-1NL4.3 in CEM-GFP reporter T and P4 (Hela-CD4-LTR-beta Gal) cell lines with a 50% effective concentration (EC50) of. 1:20000 dilution and nearly complete (98-99%) inhibition at 1:1000 dilution. It also prevented the entry of HIV-1 (IIIB) virus into P4-CCR5 cells (EC50 similar to 1:2492). Two ingredients, Aloe and Amla, inhibited the transduction of human papillomavirus type 16 (HPV-16) pseudovirus in HeLa cells at concentrations far below those that are cytotoxic and those used in the formulation. Basant was found to be totally safe according to pre-clinical toxicology carried out on rabbit vagina after application for 7 consecutive days or twice daily for 3 weeks. Basant has the potential of regressing vulvovaginal candidiasis and preventing N. gonorrhoeae, HIV and HPV infections. (C) 2008 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：180 / 185

页数：6

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