Non-equilibration of hydrostatic pressure in blebbing cells

被引:486
作者
Charras, GT [1 ]
Yarrow, JC
Horton, MA
Mahadevan, L
Mitchison, TJ
机构
[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA
[2] UCL, London Ctr Nanotechnol, London WC1E 6JF, England
[3] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA
[4] Harvard Univ, Div Engn & Appl Sci, Cambridge, MA 02138 USA
关键词
D O I
10.1038/nature03550
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Current models for protrusive motility in animal cells focus on cytoskeleton-based mechanisms, where localized protrusion is driven by local regulation of actin biochemistry(1-3). In plants and fungi, protrusion is driven primarily by hydrostatic pressure(4-6). For hydrostatic pressure to drive localized protrusion in animal cells(7,8), it would have to be locally regulated, but current models treating cytoplasm as an incompressible viscoelastic continuum(9) or viscous liquid(10) require that hydrostatic pressure equilibrates essentially instantaneously over the whole cell. Here, we use cell blebs as reporters of local pressure in the cytoplasm. When we locally perfuse blebbing cells with cortex-relaxing drugs to dissipate pressure on one side, blebbing continues on the untreated side, implying non-equilibration of pressure on scales of approximately 10 mu m and 10 s. We can account for localization of pressure by considering the cytoplasm as a contractile, elastic network infiltrated by cytosol. Motion of the fluid relative to the network generates spatially heterogeneous transients in the pressure field, and can be described in the framework of poroelasticity(11,12).
引用
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页码:365 / 369
页数:5
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