Sequence-specific detection of microRNAs by signal-amplifying ribozymes

被引：132

作者：

Hartig, JS ^{[1
]}

Grüne, I ^{[1
]}

Najafi-Shoushtari, SH ^{[1
]}

Famulok, M ^{[1
]}

机构：

[1] Univ Bonn, Kekule Inst Organ Chem & Biochem, D-53121 Bonn, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 03期

关键词：

D O I：

10.1021/ja038822u

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The rational and straightforward design of hairpin ribozymes that can be sequence-specifically induced by external oligonucleotides is described. Due to intrinsic signal amplification, their sensitivity is at least an order of magnitude increased compared to standard molecular beacons. We applied this system to the detection of microRNAs, a recently discovered class of small endogenous RNA molecules that are involved in gene regulation. We show that the cognate microRNA can reliably and sensitively be detected at low concentrations in a mix of other microRNA sequences. These probes may be useful in applications that require direct detection of minute amounts of small DNAs or RNAs. Copyright © 2004 American Chemical Society.

引用

页码：722 / 723

页数：2

共 18 条

[1] Role of microRNAs in plant and animal development [J].

Carrington, JC ;

Ambros, V .

SCIENCE, 2003, 301 (5631) :336-338

[2] PSEUDO HALF-KNOT FORMATION WITH RNA [J].

ECKER, DJ ;

VICKERS, TA ;

BRUICE, TW ;

FREIER, SM ;

JENISON, RD ;

MANOHARAN, M ;

ZOUNES, M .

SCIENCE, 1992, 257 (5072) :958-961

[3] Molecular beacons - Fluorogenic probes for living cell study [J].

Fang, XH ;

Mi, YM ;

Li, JWJ ;

Beck, T ;

Schuster, S ;

Tan, WH .

CELL BIOCHEMISTRY AND BIOPHYSICS, 2002, 37 (02) :71-81

[4] Protein-dependent ribozymes report molecular interactions in real time [J].

Hartig, JS ;

Najafi-Shoushtari, SH ;

Grüne, I ;

Yan, A ;

Ellington, AD ;

Famulok, M .

NATURE BIOTECHNOLOGY, 2002, 20 (07) :717-722

[5]

Jenne A, 1999, ANGEW CHEM INT EDIT, V38, P1300, DOI 10.1002/(SICI)1521-3773(19990503)38:9<1300::AID-ANIE1300>3.0.CO

[6]

2-Q

[7] Rapid identification and characterization of hammerhead-ribozyme inhibitors using fluorescence-based technology [J].

Jenne, A ;

Hartig, JS ;

Piganeau, N ;

Tauer, A ;

Samarsky, DA ;

Green, MR ;

Davies, J ;

Famulok, M .

NATURE BIOTECHNOLOGY, 2001, 19 (01) :56-61

[8] In vitro selection of hairpin ribozymes activated with short oligonucleotides [J].

Komatsu, Y ;

Nobuóka, K ;

Karino-Abe, N ;

Matsuda, A ;

Ohtsuka, E .

BIOCHEMISTRY, 2002, 41 (29) :9090-9098

[9] Identification of novel genes coding for small expressed RNAs [J].

Lagos-Quintana, M ;

Rauhut, R ;

Lendeckel, W ;

Tuschl, T .

SCIENCE, 2001, 294 (5543) :853-858

[10] Riboswitches control fundamental biochemical pathways in Bacillus subtilis and other bacteria [J].

Mandal, M ;

Boese, B ;

Barrick, JE ;

Winkler, WC ;

Breaker, RR .

CELL, 2003, 113 (05) :577-586

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