In vitro and in vivo two-photon luminescence imaging of single gold nanorods

被引：839

作者：

Wang, HF

Huff, TB

Zweifel, DA

He, W

Low, PS

Wei, A

Cheng, JX

机构：

[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 44期

关键词：

in vivo imaging; plasmon resonance; multiphoton; nonlinear optics;

D O I：

10.1073/pnas.0504892102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.

引用

页码：15752 / 15756

页数：5

共 32 条

[1] Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm [J].