Demonstration of cyclin D1 (Bcl-l) in mantle cell lymphoma - Enhanced staining using heat and ultrasound epitope retrieval

被引：32

作者：

Brynes, RK

McCourty, A

Tamayo, R

Jenkins, K

Battifora, H

机构：

[1] Division of Pathology, City of Hope National Medical Center, Duarte, CA

[2] Division of Pathology, City of Hope National Medical Center, Duarte

来源：

APPLIED IMMUNOHISTOCHEMISTRY | 1997年 / 5卷 / 01期

关键词：

epitope retrieval; ultrasound; cyclin D1; Bcl-1;

D O I：

10.1097/00022744-199703000-00007

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Cyclin D1/bcl-1 is overexpressed in mantle cell lymphoma. Demonstration of this nucleoprotein by immunohistochemistry may be diagnostically useful. We evaluated the recently described sonication-induced method of epitope retrieval and compared it with microwave heat-induced epitope unmasking. The strongest staining intensities and lowest background staining were achieved when both epitope unmasking methods were combined.

引用

页码：45 / 48

页数：4

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