Antibacterial and physical properties of calcium-phosphate and calcium-fluoride nanocomposites with chlorhexidine

被引：120

作者：

Cheng, Lei ^{[1
,2
]}

Weir, Michael D. ^{[1
]}

Xu, Hockin H. K. ^{[1
,3
,4
,5
]}

Kraigsley, Alison M. ^{[6
]}

Lin, Nancy J. ^{[6
]}

Lin-Gibson, Sheng ^{[6
]}

Zhou, Xuedong ^{[2
]}

机构：

[1] Univ Maryland, Sch Dent, Biomat & Tissue Engn Div, Dept Endodont Prosthodont & Operat Dent, Baltimore, MD 21201 USA

[2] Sichuan Univ, W China Sch Stomatol, State Key Lab Oral Dis, Chengdu 610064, Peoples R China

[3] Univ Maryland, Sch Med, Ctr Stem Cell Biol & Regenerat Med, Baltimore, MD 21201 USA

[4] Univ Maryland, Sch Med, Marlene & Stewart Greenebaum Canc Ctr, Baltimore, MD 21201 USA

[5] Univ Maryland Baltimore Cty, Dept Mech Engn, Baltimore, MD 21250 USA

[6] Natl Inst Stand & Technol, Div Polymers, Biomat Grp, Gaithersburg, MD 20899 USA

来源：

DENTAL MATERIALS | 2012年 / 28卷 / 05期

关键词：

Dental nanocomposite; Calcium phosphate; Calcium fluoride; Chlorhexidine; Stress-bearing; S. mutans biofilm; Caries inhibition; STREPTOCOCCUS-MUTANS; DENTAL COMPOSITES; MECHANICAL-PROPERTIES; HIGH-STRENGTH; RESTORATIONS; RELEASE; DEMINERALIZATION; BIOFILMS; DELIVERY; PLAQUE;

D O I：

10.1016/j.dental.2012.01.006

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

Objectives. Previous studies have developed calcium phosphate and fluoride releasing composites. Other studies have incorporated chlorhexidine (CHX) particles into dental composites. However, CHX has not been incorporated in calcium phosphate and fluoride composites. The objectives of this study were to develop nanocomposites containing amorphous calcium phosphate (ACP) or calcium fluoride (CaF2) nanoparticles and CHX particles, and investigate Streptococcus mutans biofilm formation and lactic acid production for the first time. Methods. Chlorhexidine was frozen via liquid nitrogen and ground to obtain a particle size of 0.62 mu m. Four nanocomposites were fabricated with fillers of: nano ACP; nano ACP + 10% CHX; nano CaF2; nano CaF2 + 10% CHX. Three commercial materials were tested as controls: a resin-modified glass ionomer, and two composites. S. mutans live/dead assay, colony-forming unit (CFU) counts, biofilm metabolic activity, and lactic acid were measured. Results. Adding CHX fillers to ACP and CaF2 nanocomposites greatly increased their antimicrobial capability. ACP and CaF2 nanocomposites with CHX that were inoculated with S. mutans had a growth medium pH > 6.5 after 3 d, while the control commercial composites had a cariogenic pH of 4.2. Nanocomposites with CHX reduced the biofilm metabolic activity by 10-20 folds and reduced the acid production, compared to the controls. CFU on nanocomposites with CHX were three orders of magnitude less than that on commercial composite. Mechanical properties of nanocomposites with CHX matched a commercial composite without fluoride. Significance. The novel calcium phosphate and fluoride nanocomposites could be rendered antibacterial with CHX to greatly reduce biofilm formation, acid production, CFU and metabolic activity. The antimicrobial and remineralizing nanocomposites with good mechanical properties may be promising for a wide range of tooth restorations with anti-caries capabilities. (C) 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

引用

页码：573 / 583

页数：11

共 57 条

[1]

American Dental Association (ADA), 2002, 1999 SURV DENT SERV

[2]

Synthesis and characterization of dimethacrylates containing quaternary ammonium functionalities for dental applications [J].