Mast cell-dependent neutrophil and mononuclear cell recruitment in immunoglobulin E-induced gastric reactions in mice

Background & Aims: Immunoglobulin (Ig) E-dependent reactions elicit an immediate response and can also result in a late-phase reaction that is characterized by the infiltration of leukocytes. This study assessed whether IgE-dependent late-phase responses can be elicited in the stomach wall of mice and examined the role of mast cells in this reaction, Methods: IgE-dependent gastric inflammation was elicited in genetically mast cell-deficient Kit(W)/Kit(W-v) mice, the congenic normal (+/+) mice, and mast cell-deficient Kit(W)/Kit(W-v) mice that had undergone local and selective reconstitution of gastric mast cell populations. Results: IgE-dependent gastric reactions were associated with mast cell degranulation and the infiltration of both neutrophils and mononuclear cells in normal mice, but no significant leukocyte infiltration was observed in mast cell-deficient Kit(W)/Kit(W-v) mice. By contrast, in mast cell-reconstituted Kit(W)/Kit(W-v) mice, IgE-dependent reactions were associated with the infiltration of neutrophils and mononuclear cells, Conclusions: These results show that late-phase reactions can occur during IgE-dependent gastric inflammation in the mouse and that the infiltration of both neutrophils and mononuclear cells that are observed during this reaction are mast cell dependent.