Aptamer-Mediated Surface-Enhanced Raman Spectroscopy Intensity Amplification

被引：101

作者：

Kim, Nam Hoon ^{[1
]}

Lee, Seung Joon ^{[1
]}

Moskovits, Martin ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

来源：

NANO LETTERS | 2010年 / 10卷 / 10期

基金：

美国国家科学基金会;

关键词：

Aptarners; surface enhanced Raman spectroscopy; AFM; nanoparticles; sensors; CHARGE-TRANSFER; SCATTERING; ADENOSINE; SILVER; SERS;

D O I：

10.1021/nl102495j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A bifunctional adenosine-sensitive double-stranded DNA aptamer was used to create and control a surface-enhanced Raman spectroscopy (SERS) hot spot between a bulk Au surface and a gold nanoparticle (Au NP) attached to the apramer via a biotin-avidin linkage The Au NP was decorated with 4-aminobenzenethiol (4-ABT), a Raman reporter molecule In the presence of adenosine, the target molecule, the SERS spectrum of 4-ABT increased in intensity by (concentration-dependent) factors as large as similar to 4, in situ, atomic force microscopy imaging showed the mean height of the Au NP-bearing aptamer to decrease by similar to 5 nm consistent with the observed SERS intensity change Because the aptamer's geometrical change is induced by one or two molecules, while the resulting SERS intensity changes involve many reporter molecules residing in the modified hot spot, the aptamer amplifies the SERS effect

引用

页码：4181 / 4185

页数：5

共 17 条

[1] THE EFFECTS OF THE INTERACTION BETWEEN RESONANCES IN THE ELECTROMAGNETIC RESPONSE OF A SPHERE-PLANE STRUCTURE - APPLICATIONS TO SURFACE ENHANCED SPECTROSCOPY [J].

ARAVIND, PK ;

METIU, H .

SURFACE SCIENCE, 1983, 124 (2-3) :506-528

[2] Correlation of Molecular Orientation and Packing Density in a dsDNA Self-Assembled Monolayer Observable with Surface-Enhanced-Raman Spectroscopy [J].

Barhoumi, Aoune ;

Zhang, Dongmao ;

Halas, Naomi J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (43) :14040-+

[3] Surface-enhanced Raman spectroscopy (SERS) for sub-micromolar detection of DNA/RNA mononucleotides [J].

Bell, Steven E. J. ;

Sirimuthu, Narayana M. S. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (49) :15580-15581

[4] Surface-enhanced Raman spectroscopy for DNA detection by nanoparticle assembly onto smooth metal films [J].

Braun, Gary ;

Lee, Seung Joon ;

Dante, Mark ;

Nguyen, Thuc-Quyen ;

Moskovits, Martin ;

Reich, Norbert .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (20) :6378-+

[5] Detection of adenosine using surface-enhanced Raman scattering based on structure-switching signaling aptamer [J].

Chen, Ji-Wei ;

Liu, Xue-Ping ;

Feng, Ke-Jun ;

Liang, Yi ;

Jiang, Jian-Hui ;

Shen, Guo-Li ;

Yu, Ru-Qin .

BIOSENSORS & BIOELECTRONICS, 2008, 24 (01) :66-71

[6] Biosensor-based on-site explosives detection using aptamers as recognition elements [J].

Ehrentreich-Foerster, Eva ;

Orgel, Dagmar ;

Krause-Griep, Andrea ;

Cech, Birgit ;

Erdmann, Volker A. ;

Bier, Frank ;

Scheller, F. W. ;

Rimmele, Martina .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (05) :1793-1800

[7] A rational approach to minimal high-resolution cross-reactive arrays [J].

Green, Eric ;

Olah, Mark J. ;

Abramova, Tatiana ;

Williams, Lance R. ;

Stefanovic, Darko ;

Worgall, Tilla ;

Stojanovic, Milan N. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (47) :15278-15282

[8] A DNA APTAMER THAT BINDS ADENOSINE AND ATP [J].

HUIZENGA, DE ;

SZOSTAK, JW .

BIOCHEMISTRY, 1995, 34 (02) :656-665

[9] Comparison of the signaling and stability of electrochemical DNA sensors fabricated from 6-or 11-carbon self-assembled monolayers [J].

Lai, Rebecca Y. ;

Seferos, Dwight S. ;

Heeger, Alan J. ;

Bazan, Guillermo C. ;

Plaxco, Kevin W. .

LANGMUIR, 2006, 22 (25) :10796-10800

[10]

Moskovits M, 2002, TOP APPL PHYS, V82, P215

← 1 2 →