Determination of disopyramide in human urine by capillary electrophoresis with electrochemiluminescence detection of tris(2,2′-bipyridyl)ruthenium(II)

被引：24

作者：

Fang, LY ^{[1
]}

Yin, XB ^{[1
]}

Sun, XH ^{[1
]}

Wang, EK ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2005年 / 537卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

capillary electrophoresis; electrochemiluminescence; disopyramide; extraction;

D O I：

10.1016/j.aca.2005.01.020

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis (CE) with electrochemiluminescence detection (ECL) of disopyramide based on tris(2,2'-bipyridyl) ruthenium(II) was described. After CE separation using a 50 mu.m i.d. uncoated capillary column and 10 mM running buffer solution at 15 kV of separation potential, the analyte was detected at the surface of platinum disk electrode (300 mu m in diameter) working electrode. Parameters influencing separation and detection were optimized in detail. Under optimal condition the detection limit was 2.5 x 10(-8) mol L-1 (S/N = 3). The method can be successfully applied for the determination of disopyramide in human urine after an easy, rapid and cheap extraction procedure. Extraction recovery rates were between 92.3 and 96.9%. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：25 / 30

页数：6

共 35 条

[1] SIMULTANEOUS DETERMINATION OF DISOPYRAMIDE AND ITS MONO-N-DEALKYLATED METABOLITE IN PLASMA BY GAS-LIQUID-CHROMATOGRAPHY [J].