Essential role of an active-site guanine in glmS ribozyme catalysis

被引：72

作者：

Klein, Daniel J.

Been, Michael D.

Ferre-D'Amare, Adrian R.

机构：

[1] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

[2] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 48期

关键词：

D O I：

10.1021/ja0768441

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.

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页码：14858 / +

页数：3

相关论文

共 19 条

[1] New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control [J].

Barrick, JE ;

Corbino, KA ;

Winkler, WC ;

Nahvi, A ;

Mandal, M ;

Collins, J ;

Lee, M ;

Roth, A ;

Sudarsan, N ;

Jona, I ;

Wickiser, JK ;

Breaker, RR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (17) :6421-6426

[2] Nucleobase catalysis in ribozyme mechanism [J].

Bevilacqua, Philip C. ;

Yajima, Rieko .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2006, 10 (05) :455-464

[3] Structural investigation of the GlmS ribozyme bound to its catalytic cofactor [J].

Cochrane, Jesse C. ;

Lipchock, Sarah V. ;

Strobel, Scott A. .

CHEMISTRY & BIOLOGY, 2007, 14 (01) :97-105

[4] General acid catalysis by the hepatitis delta virus ribozyme [J].

Das, SR ;

Piccirilli, JA .

NATURE CHEMICAL BIOLOGY, 2005, 1 (01) :45-52

[5] Evidence for preorganization of the glmS ribozyme ligand binding pocket [J].

Hampel, Ken J. ;

Tinsley, Melissa M. .

BIOCHEMISTRY, 2006, 45 (25) :7861-7871

[6] Backbone and nucleobase contacts to glucosamine-6-phosphate in the glmS ribozyme [J].

Jansen, Joshua A. ;

McCarthy, Tom J. ;

Soukup, Garrett A. ;

Soukup, Juliane K. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (06) :517-523

[7] A conformational switch controls hepatitis delta virus ribozyme catalysis [J].

Ke, AL ;

Zhou, KH ;

Ding, F ;

Cate, JHD ;

Doudna, JA .

NATURE, 2004, 429 (6988) :201-205

[8] Requirement of helix p2.2 and nucleotide g1 for positioning the cleavage site and cofactor of the glmS ribozyme [J].

Klein, Daniel J. ;

Wilkinson, Sara R. ;

Been, Michael D. ;

Ferre-D'Amare, Adrian R. .

JOURNAL OF MOLECULAR BIOLOGY, 2007, 373 (01) :178-189

[9] Structural basis of glmS ribozyme activation by glucosamine-6-phosphate [J].

Klein, Daniel J. ;

Ferre-D'Amare, Adrian R. .

SCIENCE, 2006, 313 (5794) :1752-1756

[10] Ligand requirements for glmS ribozyme self-cleavage [J].

McCarthy, TJ ;

Plog, MA ;

Floy, SA ;

Jansen, JA ;

Soukup, JK ;

Soukup, GA .

CHEMISTRY & BIOLOGY, 2005, 12 (11) :1221-1226