Characterization and application of high magnetic property chitosan particles

被引：26

作者：

An, XN

Su, ZX ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Gansu 730000, Peoples R China

[2] Nankai Univ, State Key Lab Funct Polymer Mat Adsorpt & Separat, Tianjin 300071, Peoples R China

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2001年 / 81卷 / 05期

关键词：

high magnetic property chitosan particles; aprotinin immobilization; affinity purification of trypsin;

D O I：

10.1002/app.1539

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

High magnetic property chitosan particles were prepared from chitosan coated on magnetic powder, glutaraldehyde modified, and then epichlorohydrin crosslinked. The magnetic properties, the surface, and the morphology of the magnetic particles were characterized. An optimum condition of immobilization of aprotinin on magnetic particles was obtained, then these particles were used for affinity purification of trypsin, and the condition of affinity purification was discussed. The proposed method was successfully applied to the purification of trypsin. (C) 2001 John Wiley & Sons, Inc.

引用

页码：1175 / 1181

页数：7

共 25 条

[1] Simple and broadly applicable sample preparation by use of magnetic glass particles [J].