Characterization of and host response to tyramine substituted-hyaluronan enriched fascia extracellular matrix

被引:23
作者
Chin, LiKang [1 ,2 ]
Calabro, Anthony [1 ]
Rodriguez, E. Rene [3 ]
Tan, Carmela D. [3 ]
Walker, Esteban [4 ]
Derwin, Kathleen A. [1 ]
机构
[1] Cleveland Clin, Lerner Res Inst, Dept Biomed Engn, Cleveland, OH 44195 USA
[2] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA
[3] Cleveland Clin, Dept Anat Pathol, Cleveland, OH 44195 USA
[4] Cleveland Clin, Dept Quantitat Hlth Sci, Cleveland, OH 44195 USA
基金
美国国家卫生研究院;
关键词
SMALL-INTESTINAL SUBMUCOSA; TISSUE RECONSTRUCTION; MACROPHAGE PHENOTYPE; AMPHOTERICIN-B; IN-VIVO; SCAFFOLD; REPAIR; GRAFT; FIBROBLASTS; RABBIT;
D O I
10.1007/s10856-011-4325-4
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
Naturally-occurring biomaterial scaffolds derived from extracellular matrix (ECM) have been previously investigated for soft tissue repair. We propose to enrich fascia ECM with high molecular weight tyramine substituted-hyaluronan (TS-HA) to modulate inflammation associated with implantation and enhance fibroblast infiltration. As critical determinants of constructive remodeling, the host inflammatory response and macrophage polarization to TS-HA enriched fascia were characterized in a rat abdominal wall model. TS-HA treated fascia with crosslinking had a similar lymphocyte (P = 0.11) and plasma cell (P = 0.13) densities, greater macrophage (P = 0.001) and giant cell (P < 0.0001) densities, and a lower density of fibroblast-like cells (P < 0.0001) than water treated controls. Treated fascia, with or without cross-linking, exhibited a predominantly M2 pro-remodeling macrophage profile similar to water controls (P = 0.82), which is suggestive of constructive tissue remodeling. Our findings demonstrated that HA augmentation can alter the host response to an ECM, but the appropriate concentration and molecular weight needed to minimize chronic inflammation within the scaffold remains to be determined.
引用
收藏
页码:1465 / 1477
页数:13
相关论文
共 59 条
[1]
Rotator cuff repair using an acellular dermal matrix graft: An in vivo study in a canine model [J].
Adams, Julie E. ;
Zobitz, Mark E. ;
Reach, John S., Jr. ;
an, Kai-N An ;
Steinmann, Scott P. .
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY, 2006, 22 (07) :700-709
[2]
Granulomatous reaction to injectable hyaluronic acid (Restylane) diagnosed by fine needle biopsy [J].
Al-Shraim, Mubarak ;
Jaragh, Mohammad ;
Geddie, William .
JOURNAL OF CLINICAL PATHOLOGY, 2007, 60 (09) :1060-1061
[3]
Commercially available extracellular matrix materials for rotator cuff repairs: State of the art and future trends [J].
Aurora, Amit ;
McCarron, Jesse ;
Iannotti, Joseph P. ;
Derwin, Kathleen .
JOURNAL OF SHOULDER AND ELBOW SURGERY, 2007, 16 (05) :171S-178S
[4]
Morphologic study of small intestinal submucosa as a body wall repair device [J].
Badylak, S ;
Kokini, K ;
Tullius, B ;
Simmons-Byrd, A ;
Morff, R .
JOURNAL OF SURGICAL RESEARCH, 2002, 103 (02) :190-202
[5]
Badylak S, 2003, HEART SURG FORUM, V6, pE20
[6]
The extracellular matrix as a scaffold for tissue reconstruction [J].
Badylak, SE .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2002, 13 (05) :377-383
[8]
SMALL INTESTINAL SUBMUCOSA AS A LARGE DIAMETER VASCULAR GRAFT IN THE DOG [J].
BADYLAK, SF ;
LANTZ, GC ;
COFFEY, A ;
GEDDES, LA .
JOURNAL OF SURGICAL RESEARCH, 1989, 47 (01) :74-80
[9]
Immune response to biologic scaffold materials [J].
Badylak, Stephen E. ;
Gilbert, Thomas W. .
SEMINARS IN IMMUNOLOGY, 2008, 20 (02) :109-116
[10]
Macrophage Phenotype as a Determinant of Biologic Scaffold Remodeling [J].
Badylak, Stephen F. ;
Valentin, Jolene E. ;
Ravindra, Anjani K. ;
McCabe, George P. ;
Stewart-Akers, Ann M. .
TISSUE ENGINEERING PART A, 2008, 14 (11) :1835-1842