Lighting E-coli cells as biological sensors for Cd2+

被引：22

作者：

Prachayasittikul, V

Isarankura-Na-Ayudhya, C

Bulow, L ^{[1
]}

机构：

[1] Lund Univ, Ctr Chem & Chem Engn, Dept Pure & Appl Biochem, Lund, Sweden

[2] Mahidol Univ, Fac Med Technol, Dept Clin Microbiol, Bangkok 10700, Thailand

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 16期

关键词：

cadmium determination; chimeric protein; green fluorescent protein; metal binding peptide;

D O I：

10.1023/A:1010561120720

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Whole cells of E. coli expressing a chimeric cadmium-binding peptide fused to green fluorescent protein (CdBP-GFP) were prepared and applied for the determination of cadmium. Construction of the structural gene was performed by inserting two synthetic oligonucleotides coding for four repeats of a Cd-binding peptide (His-Ser-Gln-Lys-Val-Phe) into the 5'-end of the GFPuv gene. Similarly, a hexahistidine-green fluorescent protein (his(6)GFPuv) was prepared and used as a reference in the determinations of heavy metals. The lowest concentrations of Cd, which activated the fluorescence, were 0.5 muM, 50 muM, and 0.5 mM for cells carrying CdBP(4)GFP, his(6)GFP and native GFP, respectively.

引用

页码：1285 / 1291

页数：7

共 35 条

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