Stat3-dependent induction of interleukin-3 receptor expression in leukemia inhibitory factor-stimulated M1 mouse leukemia cells

被引：7

作者：

Iwamoto, T

Senga, T

Adachi, K

Hamaguchi, M

机构：

[1] Nagoya Univ, Sch Med, Radioisotope Res Ctr, Div Med,Showa Ku, Nagoya, Aichi 4668550, Japan

[2] Nagoya Univ, Sch Med, Div Canc Biol, Nagoya, Aichi 4668550, Japan

来源：

CYTOKINE | 2004年 / 25卷 / 03期

基金：

日本学术振兴会;

关键词：

leukemia inhibitory factor; interleukin-3; receptor; Stat3; MI; transcription;

D O I：

10.1016/j.cyto.2003.10.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

M1 mouse leukemia cells differentiate to macrophages/monocytes by the stimulation of interleukin-6 (IL-6)/leukemia inhibitory factor (LIF). To identify new LIF-induced genes, we have performed representational difference analysis using M1 cells and cloned mouse interleukin-3 (IL-3) receptor beta subunit gene. The mRNA expression of both IL-3 receptor (IL-3R) alpha and beta subunits is upregulated after 1 h stimulation of LIF and remains to be elevated along the differentiation of M1 cells. This induction is almost completely suppressed in M1 cells expressing a dominant negative form of Stat3. Furthermore, we show that IL-3-induced Staff phosphorylation increases in LIF-stimulated M 1 cells. These results suggest that Stat3 may play a role in the differentiation of myeloid cells by regulating IL-3R expression. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：136 / 139

页数：4

共 18 条

[1] Analysis of the 5′ promoters for human IL-3 and GM-CSF receptor α genes [J].