A tandem segmental duplication (TSD) in green revolution gene Rht-D1b region underlies plant height variation

被引：92

作者：

Li, Yiyuan ^{[1
,2
]}

Xiao, Jianhui ^{[1
]}

Wu, Jiajie ^{[1
]}

Duan, Jialei ^{[1
,2
]}

Liu, Yue ^{[1
]}

Ye, Xingguo ^{[1
]}

Zhang, Xin ^{[1
]}

Guo, Xiuping ^{[1
]}

Gu, Yongqiang ^{[3
]}

Zhang, Lichao ^{[1
]}

Jia, Jizeng ^{[1
]}

Kong, Xiuying ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Key Lab Crop Gene Resources & Germplasm Enhanceme, Natl Key Facil Crop Gene Resources & Genet Improv, Inst Crop Sci,Minist Agr, Beijing 100081, Peoples R China

[2] China Agr Univ, Coll Biol Sci, Beijing 100094, Peoples R China

[3] USDA, ARS, Western Reg Res Ctr, Albany, CA 94710 USA

来源：

NEW PHYTOLOGIST | 2012年 / 196卷 / 01期

关键词：

copy number variation; gene expression; plant height; tandem segmental duplication (TSD); wheat (Triticum aestivum); GIBBERELLIN; PROTEIN; EVOLUTION; GENOME; WHEAT; MECHANISMS; RICE; EXPRESSION; RESISTANCE; TOLERANCE;

D O I：

10.1111/j.1469-8137.2012.04243.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Rht-D1c (Rht10) carried by Chinese wheat (Triticum aestivum) line Aibian 1 is an allele at the Rht-D1 locus. Among the Rht-1 alleles, little is known about Rht-D1c although it determines an extreme dwarf phenotype in wheat. Here, we cloned and functionally characterized Rht-D1c using a combination of Southern blotting, target region sequencing, gene expression analysis and transgenic experiments. We found that the Rht-D1c allele was generated through a tandem segmental duplication (TSD) of a > 1 Mb region, resulting in two copies of the Rht-D1b. Two copies of Rht-D1b in the TSD were three-fold more effective in reducing plant height than a single copy, and transformation with a segment containing the tandemly duplicated copy of Rht-D1b resulted in the same level of reduction of plant height as the original copy in Aibian 1. Our results suggest that changes in gene copy number are one of the important sources of genetic diversity and some of these changes could be directly associated with important traits in crops.

引用

页码：282 / 291

页数：10

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