Positive correlation of tumor necrosis factor-α early expression in myocardium and ventricular arrhythmias in rats with acute myocardial infarction

被引：27

作者：

Xiao, Hua ^{[1
]}

Chen, Zhijian ^{[1
]}

Liao, Yuhua ^{[1
]}

Cheng, Xiang ^{[1
]}

Liu, Kun ^{[1
]}

Wang, Yanfu ^{[1
]}

Wang, Min ^{[1
]}

Guo, Heping ^{[1
]}

机构：

[1] Hua Zhong Univ Sci & Technol, Lab Cardiovasc Immunol, Union Hosp,Inst Cardiol, Tong Ji Med Coll, Wuhan 430022, Hubei Province, Peoples R China

来源：

ARCHIVES OF MEDICAL RESEARCH | 2008年 / 39卷 / 03期

关键词：

myocardial infarction; tumor necrosis factor-alpha; arrhythmia; calcium channels; sudden cardiac death;

D O I：

10.1016/j.arcmed.2007.10.004

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background. Tumor necrosis factor-alpha (TNF-alpha) is found to play important roles in acute myocardial infarction (AMI). Ventricular arrhythmias arising from AMI are le ading causes of sudden cardiac death (SCD). We sought to clarify the effect of TNF-alpha early expression on ventricular arrhythmias in rats with AMI and its mechanism. Methods. Rats with AMI were induced by left anterior descending coronary branch ligation. The mRNA and protein levels of TNF-alpha in myocardium were detected by real-time fluorescent quantitative PCR, Western blotting and histochemistry. Meanwhile, electrocardiogram was recorded. Different concentrations of TNF-alpha were added to isolated rat hearts in isolated heart perfusions. Effect of TNF-alpha on intracellular Ca2+ concentration was detected by laser confocal technique. Results. In AMI rats, mRNA and protein levels of TNF-alpha were higher than control (p < 0.05), and the occurrence time of ventricular arrhythmias coincided with the secretion of TNF-alpha. TNF-alpha may cause ventricular arrhythmias in isolated rat heart perfusion models. Intracellular Ca2+ intensity may quickly be increased by TNF-alpha. Conclusions. Our results reveal the positive correlation between TNF-alpha early expression and ventricular arrhythmias in rats with AMI. This effect may be associated with the increased intracellular Ca2+ intensity caused by TNF-alpha. (c) 2008 IMSS. Published by Elsevier Inc.

引用

页码：285 / 291

页数：7

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