Immuno separation of Prion protein-enriched domains from other detergent-resistant membrane fractions, isolated from neuronal cells

被引：33

作者：

Botto, L

Masserini, M

Cassetti, A

Palestini, P

机构：

[1] Univ Milano Bicocca, Sch Med,DIMESAB, Dept Expt Environm Med & Biotechnol, I-20052 Monza, Italy

[2] Univ Milan, Bicocca, Dept Neurosci, Consorzio MIA, I-20052 Monza, Italy

来源：

FEBS LETTERS | 2004年 / 557卷 / 1-3期

关键词：

neuron; lipid raft; prion protein; PKC; GAP43;

D O I：

10.1016/S0014-5793(03)01463-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The possibility of coexistence of different subtypes of membrane lipid rafts has been investigated in cerebellar granule cells, by submitting detergent-resistant membrane fractions to immunoprecipitation. Among the proteins and lipids present in detergent-resistant fractions, almost all Prion protein, GAP43 and PKC were present in the immunoprecipitate obtained with anti-GAP43 or anti-Prion protein antibody at 4degreesC, together with a small fraction of cholesterol and sphingolipids, suggesting that they belong to a distinct subset of membranes. On the contrary, all Fyn and almost all MARCKS remained in the supernatant. Fluorescence microscopy experiments showed that Fyn and Prion protein were mostly not colocalized within a single neuron. Our results suggest that granule cells membranes contains different subtypes of detergent-resistant fractions, possibly deriving from different lipid rafts. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

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页码：143 / 147

页数：5

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