Application of an improved ELISA assay to the analysis of urinary estrogen metabolites

被引：74

作者：

Bradlow, HL

Sepkovic, DW

Klug, T

Osborne, MP

机构：

[1] Strang Canc Res Lab, New York, NY USA

[2] Immunacare Inc, Bethlehem, PA USA

来源：

STEROIDS | 1998年 / 63卷 / 7-8期

关键词：

ELISA; estrogen metabolites; urinary assay; boast cancer risk; 7-hydroxyestrone; 16; alpha-hydroxyestrone; postmenopausal;

D O I：

10.1016/S0039-128X(98)00041-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Work from Strang and other laboratories has established that the 2-/16 alpha-hydroxystrone ratio is inversely correlated with the risk for breast and cervical cancer. In order to measure these metabolites in urine samples, it is essential to have an assay for these compounds that is both sensitive and reproducible. The present paper describes such an ELISA assay, which overcomes problems that existed in prior approaches to measuring these compounds. The new ELISA procedure supplies greater sensitivity and reproducibility than earlier assay procedures. The ELISA assay has also been found to correlate well with the GC-MS procedure of Adlecreutz.

引用

页码：406 / 413

页数：8

共 26 条

[1]

Bradlow HI, 1996, J ENDOCRINOL, V150, pS259

[2]

BRADLOW HL, 1996, CANCER EPIDEM BIOMAR, V6, P145

[3]

BULBROOK RD, 1967, LANCET, V1, P519

[4]

Chen C, 1996, CANCER EPIDEM BIOMAR, V5, P727

[5]

CRANE MM, 1996, UNPUB PILOT STUDY

[6]

KABAT GC, 1997, CANCER EPIDEM BIOMAR, V6, P500

[7] MONOCLONAL ANTIBODY-BASED ENZYME-IMMUNOASSAY FOR SIMULTANEOUS QUANTITATION OF 2-ALPHA-HYDROXYESTRONE AND 16-ALPHA-HYDROXYESTRONE IN URINE [J].

KLUG, TL ;

BRADLOW, HL ;

SEPKOVIC, DW .

STEROIDS, 1994, 59 (11) :648-655

[8]

LEMON HM, 1991, CANCER, V68, P444, DOI 10.1002/1097-0142(19910715)68:2<444::AID-CNCR2820680240>3.0.CO

[9]

2-L

[10]

LEVEY S, 1950, AM J CLIN PATHOL, V20, P159

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