Mass spectrometry of ribosomes and ribosomal subunits

被引:89
作者
Benjamin, DR
Robinson, CV
Hendrick, JP
Hartl, FU
Dobson, CM
机构
[1] Univ Oxford, Oxford Ctr Mol Sci, New Chem Lab, Oxford OX1 3QT, England
[2] Mem Sloan Kettering Canc Ctr, Howard Hughes Med Inst, Cellular Biochem & Biophys Program, New York, NY 10021 USA
关键词
D O I
10.1073/pnas.95.13.7391
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Nanoflow electrospray ionization has been used to introduce intact Escherichia coli ribosomes into the ion source of a mass spectrometer. Mass spectra of remarkable quality result from a partial, but selective, dissociation of the particles within the mass spectrometer. Peaks in the spectra have been assigned to individual ribosomal proteins and to noncovalent complexes of up to five component proteins, The pattern of dissociation correlates strongly with predicted features of ribosomal protein-protein and protein-RNA interactions. The spectra allow the dynamics and state of folding of specific proteins to be investigated in the context of the intact ribosome. This study demonstrates a potentially general strategy to probe interactions within complex biological assemblies.
引用
收藏
页码:7391 / 7395
页数:5
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