Human skeletal muscle carnitine palmitoyltransferase I activity determined in isolated intact mitochondria

This study was designed to compare the activity of skeletal muscle carnitine palmitoyltransferase I (CPT I) in trained and inactive men (n = 14) and women (n = 12). CPT I activity was measured in intact mitochondria, isolated from needle biopsy vastus lateralis muscle samples (similar to 60 mg). The variability of CPT I activity determined on two biopsy samples from the same leg on the same day was 4.4, whereas it was 7.0% on two biopsy samples from the same leg on different days. The method was sensitive to the CPT I inhibitor malonyl-CoA(88% inhibition) and therefore specific for CPT I activity. The mean CPT I activity for all 26 subjects was 141.1 +/- 10.6 mu mol . min(-1) . kg wet muscle (wm)(-1) and was not different when all men vs. all women (140.5 +/- 15.7 and 142.2 +/- 14.5 mu mol . min(-1) . kg wm(-1), respectively) were compared. However, CPT I activity was significantly higher in trained vs, inactive subjects for both men (176.2 +/- 21.1 vs. 104.1 +/- 13.6 mu mol min(-1) . kg wm(-1)) and women (167.6 +/- 14.1 vs. 91.2 +/- 9.5 mu mol . min(-1) . kg wm(-1)). CPT I activity was also significantly correlated with citrate synthase activity tall subjects, r = 0.76) and maximal oxygen consumption expressed in milliliters per kilogram per minute tall subjects, r = 0.69). The results of this study suggest that CPT I activity can be accurately and reliably measured in intact mitochondria isolated from human muscle biopsy samples. CPT I activity was not affected by gender, and higher activities in aerobically trained subjects appeared to be the result of increased mitochondrial content in both men and women.