Efficiency of von Willebrand factor-mediated targeting of interleukin-8 into Weibel-Palade bodies

被引:28
作者
Bierings, R.
Van Den Biggelaar, M.
Kragt, A.
Mertens, K.
Voorberg, J.
Van Mourik, J. A.
机构
[1] Univ Amsterdam, Dept Plasma Proteins, Sanquin Res & Landsteiner Lab AMC, Amsterdam, Netherlands
[2] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Utrecht, Netherlands
[3] Univ Amsterdam, Acad Med Ctr, Dept Vasc Med, Amsterdam, Netherlands
关键词
endothelial cells; IL-8; sorting efficiency; von Willebrand factor; Weibel-Palade bodies;
D O I
10.1111/j.1538-7836.2007.02768.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: After de novo synthesis in endothelial cells, the chemokine interleukin-8 (IL-8) is targeted to endothelial cell-specific storage vesicles, the Weibel-Palade bodies (WPBs), where it colocalizes with von Willebrand factor (VWF). Objective: In this study we investigated a putative regulator function for VWF in the recruitment of IL-8 to WPBs. Methods: We performed a quantitative analysis of the entry of IL-8 into the storage system of the endothelium using pulse-chase analysis and subcellular fractionation studies. Results: Using pulse-chase analysis of IL-1 beta-stimulated human umbilical vein endothelial cells, we found that a small part of de novo synthesized IL-8 was retained in endothelial cells after 4 h. In density gradients of endothelial cell homogenates nearly equimolar amounts of VWF and IL-8 were present in subcellular fractions that contained WPBs. Furthermore, we found that IL-8 binds to immobilized VWF under the slightly acidic conditions thought to prevail in the lumen of the late secretory pathway. Conclusions: These observations indicate that the sorting efficiency of IL-8 into the regulated secretory pathway of the endothelium is tightly controlled by the entry of VWF into WPBs.
引用
收藏
页码:2512 / 2519
页数:8
相关论文
共 30 条
[1]   VESICLES AND CISTERNAE IN THE TRANS GOLGI-APPARATUS OF HUMAN-FIBROBLASTS ARE ACIDIC COMPARTMENTS [J].
ANDERSON, RGW ;
PATHAK, RK .
CELL, 1985, 40 (03) :635-643
[2]   Sorting and storage during secretory granule biogenesis: looking backward and looking forward [J].
Arvan, P ;
Castle, D .
BIOCHEMICAL JOURNAL, 1998, 332 :593-610
[3]   Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor [J].
Blagoveshchenskaya, AD ;
Hannah, MJ ;
Allen, S ;
Cutler, DF .
MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (05) :1582-1593
[4]   Quantitative analysis of von Willebrand factor propeptide release in vivo: Effect of experimental endotoxemia and administration of 1-deamino-8-D-arginine vasopressin in humans [J].
Borchiellini, A ;
Fijnvandraat, K ;
tenCate, JW ;
Pajkrt, D ;
vanDeventer, SJH ;
Pasterkamp, G ;
MeijerHuizinga, F ;
ZwartHuinink, L ;
Voorberg, J ;
vanMourik, JA .
BLOOD, 1996, 88 (08) :2951-2958
[5]   THE ACTIVATION OF HUMAN BLOOD-COAGULATION FACTOR-X ON THE SURFACE OF ENDOTHELIAL-CELLS - A COMPARISON WITH VARIOUS VASCULAR CELLS, PLATELETS AND MONOCYTES [J].
BRINKMAN, HJM ;
MERTENS, K ;
HOLTHUIS, J ;
ZWARTHUININK, LA ;
GRIJM, K ;
VANMOURIK, JA .
BRITISH JOURNAL OF HAEMATOLOGY, 1994, 87 (02) :332-342
[6]   Differential regulation of endothelial exocytosis of P-selectin and von Willebrand factor by protease-activated receptors and cAMP [J].
Cleator, JH ;
Zhu, WQ ;
Vaughan, DE ;
Hamm, HE .
BLOOD, 2006, 107 (07) :2736-2744
[7]   Von Willebrand factor targets IL-8 to Weibel-Palade bodies in an endothelial cell line [J].
de Wit, TR ;
de Leeuw, HPJC ;
Rondaij, MG ;
de Laaf, RTM ;
Sellink, E ;
Brinkman, HJ ;
Voorberg, J ;
van Mourik, JA .
EXPERIMENTAL CELL RESEARCH, 2003, 286 (01) :67-74
[8]   Real-time imaging of the dynamics and secretory behavior of Weibel-Palade bodies [J].
de Wit, TR ;
Rondaij, MG ;
Hordijk, PL ;
Voorberg, J ;
van Mourik, JA .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2003, 23 (05) :755-761
[9]   INTERLEUKIN-1 INHIBITS THE SYNTHESIS OF VONWILLEBRAND-FACTOR IN ENDOTHELIAL-CELLS, WHICH RESULTS IN A DECREASED REACTIVITY OF THEIR MATRIX TOWARD PLATELETS [J].
DEGROOT, PG ;
VERWEIJ, CL ;
NAWROTH, PP ;
DEBOER, HC ;
STERN, DM ;
SIXMA, JJ .
ARTERIOSCLEROSIS, 1987, 7 (06) :605-611
[10]   Functional and spatial segregation of secretory vesicle pools according to vesicle age [J].
Duncan, RR ;
Greaves, J ;
Wiegand, UK ;
Matskovich, I ;
Bodammer, G ;
Apps, DK ;
Shipston, MJ ;
Chow, RH .
NATURE, 2003, 422 (6928) :176-180