Induction and detection of human mesenchymal stem cell migration in the 48-well reusable transwell assay

被引：38

作者：

Rüster, B ^{[1
]}

Grace, B ^{[1
]}

Seitz, O ^{[1
]}

Seifried, E ^{[1
]}

Henschler, R ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Inst Transfus Med & Immune Hematol, German Red Cross, Blood Donat Serv, D-60528 Frankfurt, Germany

来源：

STEM CELLS AND DEVELOPMENT | 2005年 / 14卷 / 02期

关键词：

D O I：

10.1089/scd.2005.14.231

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The induction and detection of chemotactic migration in mesenchymal stem cells (MSC) are complicated by their adherent nature. We describe here the experimental details for inducing the transmigration of MSC seeded in microchemotaxis chambers and reliably identifying the translocated cells. A combination of 8-mu m pore-sized membranes and a two-step staining procedure resulted in the detection of MSC migration against a gradient of human plasma which plateaued after 4 h. Microscopic enumeration of the transmigrated cells clearly distinguished contaminating nonmigrated cells from transmigrated cells. Chemokinetic and chemotactic effects could be separated and were influenced by precoated fibronectin or vitronectin. Thus, the technique described allows rapid and reliable induction and determination of migration in adherent MSC.

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页码：231 / 235

页数：5

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