Emulsan quantitation by Nile red quenching fluorescence assay

被引：25

作者：

Castro, GR

Larson, BK

Panilaitis, B

Kaplan, DL

机构：

[1] Tufts Univ, Sch Engn, Dept Biomed Engn, Medford, MA 02155 USA

[2] Planto Piloto Proc Ind Microbiol, RA-4000 San Miguel De Tucuman, Argentina

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2005年 / 67卷 / 06期

关键词：

D O I：

10.1007/s00253-004-1849-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Nile red fluorescent technique to quantify 20 200 mu g ml(-1) of emulsan was developed. Nile red dissolved in DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm(-1) M-1. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG-1.

引用

页码：767 / 770

页数：4

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